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| + | <h2 style="border-bottom: 5px solid #005b04;padding-left: 1.0cm;">Research on screening system. Decision to work with kit from iGEM Team Austin, Texas 2014</h2> | ||
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| + | <h2 style="border-bottom: 5px solid #005b04;padding-left: 1.0cm;">Definition of incubation conditions</h2> | ||
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| + | <p align="justify" style="padding-left: 1.0cm; padding-right: 1.0cm; font-size:16px;"><b style="color:#005b04; ">Arrival and Preparation of Testkit with DNA from Texas</b><br/> | ||
| + | Recovery of plasmids in TRIS buffer pH 8.5, followed by transformation in BL21 DE3 gold, LB solid plates with Gentamycin 30µg/ml. | ||
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Revision as of 18:17, 15 October 2016
Lab Book
To express a photocaged protease in our case subtilisin E, we developed three lab strategies. Their milestones can be seen below in three different timelines, one for every sub-division. Details about reaching these milestones will become visible, if you click on them. On the page “Project Idea” the theory beneath our lab work is described and on the subpage “Protocols & Methods” under “Lab” the exact protocols can be seen, if you would like to repeat the experiments.
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Research on screening system. Decision to work with kit from iGEM Team Austin, Texas 2014
Definition of incubation conditions
Arrival and Preparation of Testkit with DNA from Texas
Recovery of plasmids in TRIS buffer pH 8.5, followed by transformation in BL21 DE3 gold, LB solid plates with Gentamycin 30µg/ml.
