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- by 2A technical : BBa_B0034+BBa_I0500 - by 3A technical : Bba_K1951005 and Bba_K880005 digested the 07.18.2016
- 98°C 30s, (98°C 10s, 60°C 30s, 72°C 1min)*30, 72°C 2min. - 98°C 30s, (98°C 10s, 72°C 1min)*30, 72°C 2min.
- Buffer Borate 0.05M pH9 ( ajusted with NaOH)
- L-Lysine 0.01M
- DEEMM 0.2M
- Hexamethylenediamine 0.01M
1 (Lysine): Borate 180µL, Methanol 60µL, H2O ppi 47µL, Lysine 30µL, DEEMM 3µL
2 (Hexamethylenediamine): Borate 180µL, Methanol 60µL, H2O ppi 47µL, Hexamethylenediamine 30µL, DEEMM 3µL
3 (Negative temoin): Borate 180µL, Methanol 60µL, H2O ppi 77µL, DEEMM 3µL
Heat to 70°C during 2 hours
NB : we wanted to transform a cadA mutant from KEIO bank so we can't use this biobrick in pSB1K3.
NB : we wanted to transform a cadA mutant from KEIO bank so we can't use this biobrick in pSB1K3.
- Bba_K1951004 + RBS (X/P) Bba_K1951001 in pSB1K3(E/P) at 16°C overnight - RBS Bba_K1951002(E/S) and RBS Bba_K1951003(X/P) in pSB1K3(E/P) at 16°C overnight
