Difference between revisions of "Team:Aix-Marseille/Description"

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==Overview==
 
==Overview==
The main goal of our project is to concentrate the platinum as much as possible.
 
 
The first step relies on the affinity of small organic molecules, siderophores, to bind solubilized Platinum atoms and thus favor the further solubilisation of more platinum compounds. We accomplish this by inserting a plasmid containing an operon with the four enzymes (Des A, Des B, Des C, Des D) necessary to synthesise our siderophore - Desferrioxamine B, into E. coli.
 
 
As a second level of concentrating the platinum even more, we plan to use the principle of biosorption. A modified fliC protein complex will be cloned into E. coli and enable the flagella of the bacterium so that it can bind platinum atoms. This specificity will be possible thanks to a recently discovered peptide that will be inserted into the sequence of the fliC. The benefit of using the biosorption is to obtain nanoparticles of platinum, a highly valuable form of the metal.
 
 
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Revision as of 13:42, 19 October 2016