Chloroplast (Talk | contribs) |
Chloroplast (Talk | contribs) |
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<div id="st-trigger-effects"> | <div id="st-trigger-effects"> | ||
− | <button data-effect="st-effect-1" class="button button--rayen button--border button--round-m" data-text="More" style="z-index: 100"> | + | <button data-effect="st-effect-1" class="button button--rayen button--border button--round-m" data-text="More" style="left: -83px; font-size: 50px; z-index: 100"> |
<span><img src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png"></span> | <span><img src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png"></span> | ||
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− | <a><h1><a href="https://2016.igem.org/Team:NUS_Singapore" style="color: black; font-weight: lighter;">iGEM 2016 | + | <a><h1><a href="https://2016.igem.org/Team:NUS_Singapore" style="color: black; font-weight: lighter;">iGEM 2016 NUS_SINGAPORE</a></h1></a> |
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− | <nav class="st-menu st-effect-1 list-group panel" id="menu-1" style="overflow: auto; z-index: 100; background-color: | + | <nav class="st-menu st-effect-1 list-group panel" id="menu-1" style="overflow: auto; z-index: 100; background-color: #E59500;"> |
− | <span><img src="https://static.igem.org/mediawiki/2016/1/1e/T--NUS_Singapore--practice.png" style="width: 30px; height: auto; top: | + | <span><img src="https://static.igem.org/mediawiki/2016/1/1e/T--NUS_Singapore--practice.png" style="width: 30px; height: auto; top: 35px; left: 35px; position: absolute;"><h4 style=" left: 75px; top: 25px; position: absolute; font-size: 17px; color: white;">NUS_SINGAPORE</h4></span> |
<ul> | <ul> | ||
<li><a class="icon icon-home" id="menu-item-1" href="https://2016.igem.org/Team:NUS_Singapore">Home</a></li> | <li><a class="icon icon-home" id="menu-item-1" href="https://2016.igem.org/Team:NUS_Singapore">Home</a></li> | ||
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<a href="https://2016.igem.org/Team:NUS_Singapore/Project/Wet_Lab" class="list-group-item" style="color: rgb(179, 179, 179);">Overview</a> | <a href="https://2016.igem.org/Team:NUS_Singapore/Project/Wet_Lab" class="list-group-item" style="color: rgb(179, 179, 179);">Overview</a> | ||
− | <a href="https://2016.igem.org/Team:NUS_Singapore/Model" class="list-group-item" style="color: rgb(179, 179, 179);">Modelling</a> | + | |
+ | <a href="https://2016.igem.org/Team:NUS_Singapore/Model" class="list-group-item" style="color: rgb(179, 179, 179);">Modelling</a> | ||
</div> | </div> | ||
</div> | </div> | ||
</li> | </li> | ||
+ | <li><a class="icon icon-notebook" id="menu-item-9" onclick="growDiv('9')" href="https://2016.igem.org/Team:NUS_Singapore/Notebook">RIOT Sensor | ||
+ | <span><img id="sidebar-arrow-9" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | ||
+ | <div class="grow" id="grow-9"> | ||
+ | <div class='measuringWrapper'> | ||
+ | <a href="https://2016.igem.org/Team:NUS_Singapore/Description" class="list-group-item" style="color: rgb(179, 179, 179);">Description</a> | ||
+ | <a href="https://2016.igem.org/Team:NUS_Singapore/Proof" class="list-group-item" style="color: rgb(179, 179, 179);">Proof of Concept</a> | ||
+ | <a href="https://2016.igem.org/Team:NUS_Singapore/Demonstrate" class="list-group-item" style="color: rgb(179, 179, 179);">Demonstration</a> | ||
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+ | </div> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTResponder">RIOT Responder</a></li> | ||
+ | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTInvader">RIOT Invader</a></li> | ||
<li><a class="icon icon-practice" onclick="growDiv('3')" id="menu-item-3" href="#">Human Practices | <li><a class="icon icon-practice" onclick="growDiv('3')" id="menu-item-3" href="#">Human Practices | ||
<span><img id="sidebar-arrow-3" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"><span></a> | <span><img id="sidebar-arrow-3" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"><span></a> | ||
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− | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations" class="list-group-item" style="color: rgb(179, 179, 179);"> | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#MELBOURNE" class="list-group-item" style="color: rgb(179, 179, 179);">MELBOURNE</a> |
− | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations" class="list-group-item" style="color: rgb(179, 179, 179);"> | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKUST" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKUST</a> |
− | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations" class="list-group-item" style="color: rgb(179, 179, 179);"> | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKU" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKU</a> |
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</div> | </div> | ||
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</li> | </li> | ||
+ | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Parts">Parts Registry</a></li> | ||
<li><a class="icon icon-safety" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Safety">Safety</a></li> | <li><a class="icon icon-safety" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Safety">Safety</a></li> | ||
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</li> | </li> | ||
<li><a class="icon icon-acknowledgement" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Attributions">Attributions</a></li> | <li><a class="icon icon-acknowledgement" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Attributions">Attributions</a></li> | ||
+ | <li><a class="icon icon-cup" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Medals">Medals</a></li> | ||
</ul> | </ul> | ||
</nav> | </nav> | ||
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<section style="background: black; position: relative; display: inline-block; overflow: hidden; height: 158%; width: 100%; padding-bottom: 3em; margin-bottom: -0.4em;"> | <section style="background: black; position: relative; display: inline-block; overflow: hidden; height: 158%; width: 100%; padding-bottom: 3em; margin-bottom: -0.4em;"> | ||
<img class="static" src="https://static.igem.org/mediawiki/2016/8/84/T--NUS_Singapore--banner.jpg" alt="Background" style="z-index: -1; position: fixed; opacity: 1; margin-top: 8%; width:100%; height: auto;" /> | <img class="static" src="https://static.igem.org/mediawiki/2016/8/84/T--NUS_Singapore--banner.jpg" alt="Background" style="z-index: -1; position: fixed; opacity: 1; margin-top: 8%; width:100%; height: auto;" /> | ||
− | <img class="static" src="https://static.igem.org/mediawiki/2016/f/f4/T--NUS_Singapore--Title_pic.png" alt="Background" style="z-index: 1; position: absolute; opacity: 1; margin-top: 8%;" /> | + | <img class="static" src="https://static.igem.org/mediawiki/2016/f/f4/T--NUS_Singapore--Title_pic.png" alt="Background" style="z-index: 1; position: absolute; opacity: 1; margin-top: 8%; width: 100%; height: auto;" /> |
<img class="static" src="https://static.igem.org/mediawiki/2016/8/84/T--NUS_Singapore--banner.jpg" alt="Background" style="opacity: 1; margin-top: 8%;" /> | <img class="static" src="https://static.igem.org/mediawiki/2016/8/84/T--NUS_Singapore--banner.jpg" alt="Background" style="opacity: 1; margin-top: 8%;" /> | ||
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<section id="canvas" class="interactive-points interactive-points--alter ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack" id="interactive-2" tabindex="0" style="background: rgb(22,55,72);"> | <section id="canvas" class="interactive-points interactive-points--alter ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack" id="interactive-2" tabindex="0" style="background: rgb(22,55,72);"> | ||
− | <img class="static" src="https://static.igem.org/mediawiki/2016/7/72/T--NUS_Singapore--Background2.png" alt="Background" style="opacity: 1; z-index: 0; width: 100%; height: 100%;" /> | + | <img class="static canvas_parts" src="https://static.igem.org/mediawiki/2016/7/72/T--NUS_Singapore--Background2.png" alt="Background" style="opacity: 1; z-index: 0; width: 100%; height: 100%;" /> |
− | <div class="CD44v6" > | + | <div class="CD44v6 canvas_parts" > |
<img id="CD44v6" class="static" src="https://static.igem.org/mediawiki/2016/5/5a/T--NUS_Singapore--CD44v6.png" style="position: absolute; width: auto; height: 100%; top: 0; left: 0; z-index: 1; opacity: 1;" /> | <img id="CD44v6" class="static" src="https://static.igem.org/mediawiki/2016/5/5a/T--NUS_Singapore--CD44v6.png" style="position: absolute; width: auto; height: 100%; top: 0; left: 0; z-index: 1; opacity: 1;" /> | ||
− | <p id="CD44v6_text" class="CD44v6_text" style="position: absolute; width: 38%; height: auto; top: | + | <p id="CD44v6_text" class="CD44v6_text" style="position: absolute; width: 38%; height: auto; top: 30%; right: 80px; text-align: justify; color: white; font-size: 1.2vw; z-index: 1; display: none;"> |
CD44 is a cell membrane protein involved in normal cell function (cell-cell and cell-matrix adhesion). However, one notable isoform, CD44v6, seems to play a major role in cancer progression, facilitating cell migration and invasion and is commonly upregulated on the surface of cancer cells. The RIOT system uses CD44v6 as a spatial marker, and recognition of this protein via a CD44v6 specific antibody allows anchoring of the engineered bacteria on the surface, triggering the expression of invasin and LLO for subsequent invasion. | CD44 is a cell membrane protein involved in normal cell function (cell-cell and cell-matrix adhesion). However, one notable isoform, CD44v6, seems to play a major role in cancer progression, facilitating cell migration and invasion and is commonly upregulated on the surface of cancer cells. The RIOT system uses CD44v6 as a spatial marker, and recognition of this protein via a CD44v6 specific antibody allows anchoring of the engineered bacteria on the surface, triggering the expression of invasin and LLO for subsequent invasion. | ||
<p> | <p> | ||
</div> | </div> | ||
− | <div class="Cancer_cell_body"> | + | <div class="Cancer_cell_body canvas_parts"> |
<img id="Cancer_cell_body" class="static" src="https://static.igem.org/mediawiki/2016/7/73/T--NUS_Singapore--Cancer_cell_body.png" style="position: absolute; width: auto; height: 100%; top: 0; left: 0; z-index: 2; opacity: 1;" /> | <img id="Cancer_cell_body" class="static" src="https://static.igem.org/mediawiki/2016/7/73/T--NUS_Singapore--Cancer_cell_body.png" style="position: absolute; width: auto; height: 100%; top: 0; left: 0; z-index: 2; opacity: 1;" /> | ||
<img id="Lactate" class="static" src="https://static.igem.org/mediawiki/2016/0/04/T--NUS_Singapore--Lactate.png" style="position: absolute; width: auto; height: 100%; top: 0; left: 0; z-index: 0.5; opacity: 1;" /> | <img id="Lactate" class="static" src="https://static.igem.org/mediawiki/2016/0/04/T--NUS_Singapore--Lactate.png" style="position: absolute; width: auto; height: 100%; top: 0; left: 0; z-index: 0.5; opacity: 1;" /> | ||
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− | <div class="Invasin_and_LLO"> | + | <div class="Invasin_and_LLO canvas_parts"> |
<img id="Invasin_and_LLO" class="static" src="https://static.igem.org/mediawiki/2016/0/06/T--NUS_Singapore--Invasin_and_LLO.png" style="position: absolute; width: auto; height: 100%; top: 0; right: 60%; z-index: 3; opacity: 1;" /> | <img id="Invasin_and_LLO" class="static" src="https://static.igem.org/mediawiki/2016/0/06/T--NUS_Singapore--Invasin_and_LLO.png" style="position: absolute; width: auto; height: 100%; top: 0; right: 60%; z-index: 3; opacity: 1;" /> | ||
<p style="position: absolute; width: 35%; height: auto; top: 25%; right: 15%; text-align: justify; color: white; font-size: 1.3vw; font-f z-index: 3; "> | <p style="position: absolute; width: 35%; height: auto; top: 25%; right: 15%; text-align: justify; color: white; font-size: 1.3vw; font-f z-index: 3; "> | ||
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− | <div class="Quorum_Sensing"> | + | <div class="Quorum_Sensing canvas_parts"> |
<img id="Quorum_Sensing" class="static" src="https://static.igem.org/mediawiki/2016/4/42/T--NUS_Singapore--Quorum_Sensing.png" style="position: absolute; width: auto; height: 100%; top: 0; right: 0; z-index: 4; opacity: 1;" /> | <img id="Quorum_Sensing" class="static" src="https://static.igem.org/mediawiki/2016/4/42/T--NUS_Singapore--Quorum_Sensing.png" style="position: absolute; width: auto; height: 100%; top: 0; right: 0; z-index: 4; opacity: 1;" /> | ||
<p id="Quorum_Sensing_text" class="Quorum_Sensing_text" style="position: absolute; width: 55%; height: auto; top: 25%; left: 180px; text-align: justify; color: white; font-size: 1.3vw; z-index: 4; display: none;"> | <p id="Quorum_Sensing_text" class="Quorum_Sensing_text" style="position: absolute; width: 55%; height: auto; top: 25%; left: 180px; text-align: justify; color: white; font-size: 1.3vw; z-index: 4; display: none;"> | ||
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<section style="text-align: justify; color: rgb(154, 155, 155); margin-left: 7%; margin-right: 7%; margin-top: 5%; margin-bottom: 5%; font-size: 12pt;"> | <section style="text-align: justify; color: rgb(154, 155, 155); margin-left: 7%; margin-right: 7%; margin-top: 5%; margin-bottom: 5%; font-size: 12pt;"> | ||
− | <h2 style="text-align: center; color: white;"> | + | <h2 style="text-align: center; color: white;"> WELCOME TO NUS_Singapore iGEM 2016 PAGE! </h2> |
− | <p> | + | <p>Biomolecule delivery systems are often plagued by problems such as non-specific targeting and low bioavailability. We sought to design a novel system that can sense and respond to specific stimuli present in the microenvironment of pathogenic cells. We engineered a dual-sensor bacterium that can sense increased metabolite levels in its microenvironment and then respond by delivering biomolecules into target cells. As a proof-of-concept, we engineered<span style="font-style: italic;"> Escherichia coli </span>to detect increased level of lactate in biological fluid, then respond by attaching itself to a cancer cell marker, and subsequently release biomolecules into the cell. A biosafety kill switch will be activated when there is insufficient lactate present, thus minimizing non-specific targeting.</p> |
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− | <p>Our | + | <p>Our proposed system has the flexibility to be engineered to detect other metabolites by changing the gene promoter and also detect different cell types by targeting other cell receptors. In addition, the modular nature of our system also allows part of the lactate sensing mechanism to be used as a diagnostic kit, especially for detecting for elevated levels of lactate in biological fluids such as blood or serum from patients with suspected cases of sepsis or lactic acidosis. This method of detection can be carried out without any specialized equipment or impoverished areas. </p> |
</section> | </section> | ||
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</ul> | </ul> | ||
</section> | </section> | ||
+ | <a href="#" class="go-top" style="color: white;">Go To Top</a> | ||
</div><!-- /st-pusher --> | </div><!-- /st-pusher --> | ||
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Latest revision as of 16:39, 19 October 2016
WELCOME TO NUS_Singapore iGEM 2016 PAGE!
Biomolecule delivery systems are often plagued by problems such as non-specific targeting and low bioavailability. We sought to design a novel system that can sense and respond to specific stimuli present in the microenvironment of pathogenic cells. We engineered a dual-sensor bacterium that can sense increased metabolite levels in its microenvironment and then respond by delivering biomolecules into target cells. As a proof-of-concept, we engineered Escherichia coli to detect increased level of lactate in biological fluid, then respond by attaching itself to a cancer cell marker, and subsequently release biomolecules into the cell. A biosafety kill switch will be activated when there is insufficient lactate present, thus minimizing non-specific targeting.
Our proposed system has the flexibility to be engineered to detect other metabolites by changing the gene promoter and also detect different cell types by targeting other cell receptors. In addition, the modular nature of our system also allows part of the lactate sensing mechanism to be used as a diagnostic kit, especially for detecting for elevated levels of lactate in biological fluids such as blood or serum from patients with suspected cases of sepsis or lactic acidosis. This method of detection can be carried out without any specialized equipment or impoverished areas.