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| − | </nav>
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| − | </header>
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| − | <!-- end hearo section -->
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| − | <div class="container-fluid">
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| − | <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">Parts</div>
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| − | <!-- start section 1 -->
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| − | <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-6 left">
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| − | <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div>
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| − | </h2></span></span>
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| − | <div class="space30"></div>
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| − | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:
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| − | <ul><br/>
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| − | <li>-T7 promoter with RBS (Bba_K525998) and optimized Laccase from <i>T.Thermophilus</i> (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)</li><br/>
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| − | <li>-T7 promoter with RBS and optimized Laccase from <i>T.Thermophilus</i>, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)</li>
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| − | </ul>
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| − | </div></p></span>
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| − | <div class="col-md-5 col-md-offset-1 col-sm-offset-1 space30">
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| − | <br/><br/><br/><br/><br/><br/><br/><br/><br/>
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| − | <img src="https://static.igem.org/mediawiki/2015/3/35/Our.png" class="img-responsive" width="475" height="325">
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| − | </div>
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| − | </div>
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| − | <div class="space30"></div>
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| − | </section>
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| − | <div class="clearfix"></div>
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| − | <!-- start section 2 -->
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| − | <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-6 left">
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| − | <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks we designed</div>
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| − | </h2></span></span>
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| − | <div class="space30"></div>
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| − | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:<br/>
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| − | <ul>
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| − | <li>-Cytochrome C <i>Synechocystis sp</i> (BBa_K1601001), a cyanobacteria that suggests a cytochrome c </li><br/>
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| − | <li>-Cytochrome C of <i>S.oneidensis</i> (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.</li><br/>
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| − | <li>-Heme A from <i>E.coli</i> K12 (BBa_K1601002)that enables the heme overproduction</li><br/>
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| − | <li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the <i>S.oneidensis</i> CytC translocation into the periplasm using TAT system (BBa_S05319)</li><br/>
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| − | <li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the <i>Synechocystis sp</i> CytC translocation into the periplasm using TAT system (BBa_S05320)</li><br/>
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| − | </ul>
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| − | </div></p></span>
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| − | </div>
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| − | <div class="col-md-5 col-md-offset-1 col-sm-offset-1 space30">
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| − | <br/><br/><br/><br/><br/><br/>
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| − | <img src="https://static.igem.org/mediawiki/2015/c/cd/Idqfgfg.png" class="img-responsive" width="1000" height="400">
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| − | </div>
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| − | </div>
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| − | <div class="space30"></div>
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| − | </div>
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| − | </section>
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| − | <!-- start section 3 -->
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| − | <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-6 left">
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| − | <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle"> Linkers we designed</div>
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| − | </h2></span></span>
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| − | <div class="space30"></div>
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| − | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We designed some linker to facilitate the meeting of the laccase and the cytochrom C
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| − | </p></span></div>
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| − | <p class="space20"><div align="justify"><span style ="font-family:Courier New">
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| − | <ul>
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| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>S.oneidensis (BBa_K1601008)</i></li> <br/>
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| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>P.denitrificans (BBa_K1601011)</i></li><br/>
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| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) <i>T.Thermophilus</i> and CytC <i>Synechocystis sp(BBa_K1601013)</i></li><br/>
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| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>P.denitrificans (BBa_K1601009)</i></li><br/>
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| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>Synechocystis sp (BBa_K1601010)</i></li><br/>
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| − | <li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>S.oneidensis(BBa_K1601012)</i></li>
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| − | </ul> </div></p></span>
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| − | </div>
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| − | <div class="col-md-5 col-md-offset-1 col-sm-offset-1 space30">
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| − | <br/><br/><br/><br/><br/><br/>
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| − | <img src="https://static.igem.org/mediawiki/2015/7/78/Idqfj%2Cg.png" class="img-responsive" width="600" height="1200">
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="space30"></div>
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| − | </div>
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| − |
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| − | </section>
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| − | <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
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| − |
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-6 left">
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| − | <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div>
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| − | </h2></span></span>
| |
| − | <div class="space30"></div>
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| − | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:<br/>
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| − | <ul>
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| − | <li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the S. oneidensis CytC translocation into the periplasm using TAT system</li>
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| − | <li>-T7 promoter with RBS and Laccase E.coli (Bba_K863006) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601003)</li>
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| − | <li>-T7 promoter with RBS and Laccase T.Thermophilus (Bba_K863011) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601004)</li>
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| − | <li>-Cytochrome C Heme Lyase (CCHL)(BBa_K1601005)</li>
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| − | <li>-Yeast signal CXXCH (BBa_K1601006)</li>
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| − | <li>-Cytochrome C P.Denitrificans (BBa_K1601007)</li>
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| − | <li>-Cytochrome C Synechocystis sp - His Tag (BBa_S05314)</li>
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| − | <li>-Cytochrome C S.oneidensis - HisTag (BBa_S05315)</li>
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| − | </ul>
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| − | </div></p></span>
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| − |
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| − |
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| − | </div>
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| − | <div class="col-md-5 col-md-offset-1 col-sm-offset-1 space30">
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| − | <br/><br/><br/><br/><br/>
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| − | <img src="https://static.igem.org/mediawiki/2015/6/60/Qsffff.png" class="img-responsive" width="475" height="325">
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| − | </div>
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| − | </div>
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| − | <div class="space30"></div>
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| − | </div>
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| − | </section>
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| − | <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
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| − |
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-6 left">
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| − | <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div>
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| − | </h2></span></span>
| |
| − | <div class="space30"></div>
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| − | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:<br/>
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| − | <ul>
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| − | <li>Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)</li>
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| − | <li>Cytochrome C P.Denitrificans - His Tag (BBa_S05317)</li>
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| − | <li>Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)</li>
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| − | <li>T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)</li>
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| − | </ul>
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| − | </div></p></span>
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| − | </div>
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| − | <div class="col-md-5 col-md-offset-1 col-sm-offset-1 space30">
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| − | <br/><br/><br/><br/><br/>
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| − | <img src="https://static.igem.org/mediawiki/2015/a/a7/Qsffqfqdf.png" class="img-responsive" width="475" height="325">
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| − | </div>
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| − | </div>
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| − | <div class="space30"></div>
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| − | </div>
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| − |
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| − | </section>
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| − | <div class="clearfix"></div>
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| − |
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| − | </section>
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| − | <!-- start social section -->
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| − | <section style="padding:30px 0px;" class="arrow_box" id="ethics">
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-12 text-center">
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| − | <h1 class="blue"><div align ="center">MORE INFORMATION ON FACEBOOK !</div></h1>
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| − | <ul class="list-inline space80 icon">
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| − | <li><div align ="center"><a href="https://www.facebook.com/iGEM.AMU?fref=ts"><img src="https://static.igem.org/mediawiki/2015/e/ed/FacebookAMU.png" width="200" height="200"" class="img-grey mautomargin"></a></li></div>
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| − | </ul>
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| − |
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | </section>
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| − | <div class="clearfix"></div>
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| − | <!-- start sponsors section -->
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| − | <section style="padding:50px 0px;" class="arrow_box-2">
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| − |
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-12 text-center">
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| − | <h2 class="gray">SPONSORS</h2>
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| − | <ul class="list-inline space30 icon">
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| − | <li><a href="https://www.corning.com/worldwide/en/products/life-sciences.html">
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| − | <img src="https://static.igem.org/mediawiki/2015/8/87/Corninglogo.jpg" width="200" align="center"
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| − | class="img-grey mautomargin"></a></li>
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| − | <li><a href="http://www.crous-aix-marseille.fr/social/fsdie-social">
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| − | <img src="https://static.igem.org/mediawiki/2015/1/18/KlP5G8h.jpg"
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| − | class="img-grey mautomargin" width="200" align="center"></a></li>
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| − | <li><a href="http://www.cnrs.fr/">
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| − | <img src="https://static.igem.org/mediawiki/2015/8/8e/CNRSlogo.jpg"
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| − | width="200" class="img-grey mautomargin" align="center"></a></li>
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| − | <li><a href="https://www.qiagen.com/fr/">
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| − | <img src="https://static.igem.org/mediawiki/2015/f/f1/Qialogo.jpg" width="200" align="center"
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| − | class="img-grey mautomargin"></a></li>
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| − | <li><a href="http://www.univ-amu.fr/">
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| − | <img src="https://static.igem.org/mediawiki/2015/3/38/EcfFf9Kw.png"
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| − | class="img-grey mautomargin" width="200" align="center" ></a></li>
| |
| − | <li><a href="http://polytech.univ-amu.fr/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/1/10/ILFZ36R.png"
| |
| − | class="img-grey mautomargin" width="200" align="center"></a></li>
| |
| − | <li><a href="http://www.grandluminy.com/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/6/66/GandLlogo.jpg" width="200" align="center"
| |
| − | class="img-grey mautomargin"></a></li>
| |
| − | <li><a href="https://www.eppendorf.com/FR-fr/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/d/db/Eppendorflogo.jpg" width="200" align="center"
| |
| − | class="img-grey mautomargin"></a></li>
| |
| − | <li><a href="http://www.dutscher.com/frontoffice/home">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/1/10/Deutcherflogo.jpg" width="200" align="center"
| |
| − | class="img-grey mautomargin"></a></li>
| |
| − | <li><a href="http://www.mn-net.com/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/1/1b/MNlogo.jpg" width="200" align="center"
| |
| − | class="img-grey mautomargin"></a></li>
| |
| − | <li><a href="https://fr.groupeonet.com/Onet-Proprete-et-Services">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/3/3d/5tokNzS.jpg" width="200" align="center"
| |
| − | class="img-grey mautomargin"></a></li>
| |
| − | <li><a href="http://www3.gehealthcare.fr/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/b/bd/GEHlogo.jpg" width="200"
| |
| − | class="img-grey mautomargin" align="center" ></a></li>
| |
| − | <li><a href="https://www.sigmaaldrich.com/france.html">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/4/41/PUNXaNj.jpg" align="center"
| |
| − | class="img-grey mautomargin" width="200"></a></li>
| |
| − | <li><a href="http://www.starlab.de/int/?l=5">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/1/13/StarLablogo.jpg" align="center"
| |
| − | class="img-grey mautomargin" width="200" height="100"></a></li>
| |
| − | <li><a href="http://fr.ambafrance-us.org/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/2/2b/M3ieC2R.jpg" align="center"
| |
| − | class="img-grey mautomargin" width="200" height="100"></a></li>
| |
| − | <li><a href="https://www.neb.com/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/a/a9/Ll3uagr.jpg" align="center"
| |
| − | class="img-grey mautomargin" width="200"></a></li>
| |
| − | <li><a href="http://sciences.univ-amu.fr/">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/4/4a/FtFgEFj.jpg" align="center"
| |
| − | class="img-grey mautomargin" width="200"></a></li>
| |
| − | <li><a href="https://eu.idtdna.com/site">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/d/d9/IDTlogo.jpg" align="center"
| |
| − | class="img-grey mautomargin" width="200"></a></li>
| |
| − | </ul>
| |
| − |
| |
| − |
| |
| − | </div>
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| − |
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| − | </div>
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| − | <ul class="f-menu space40">
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| − | <li><a href="/Team:Aix-Marseille">Home</a></li>
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| − | <li><a href="https://2015.igem.org/Main_Page">iGEM 2015</a></li>
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| − | </ul>
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| − | </div>
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| − | <div class="col-md-4">
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| − | <h3>Contact Us</h3>
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| − | <br/>
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| − | <div class="col-md-4">
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| − | <div class="f-text">
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| − | <p class="fsize18 space10">Chew figth project, for the iGEM competition. See you soon in Boston !</p>
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