Difference between revisions of "Team:Aix-Marseille/Composite Part"
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<p>This part is composed of 3 subparts : | <p>This part is composed of 3 subparts : | ||
<ul> | <ul> | ||
| − | <li> | + | <li>Bba_I0500 : Ribosome Binding Site</li> |
| − | <li>pARA/araC inductible promoter</li> | + | <li>Bba_B0034 : pARA/araC inductible promoter</li> |
| − | <li>Lysine decarboxylase</li> | + | <li>Bba_K1951000 : Lysine decarboxylase</li> |
</ul> | </ul> | ||
</p> | </p> | ||
| Line 37: | Line 37: | ||
<ul> | <ul> | ||
<li>BBa_K1951005: flagelln C</li> | <li>BBa_K1951005: flagelln C</li> | ||
| − | <li> | + | <li>BBa_K880005: Strong promoter, strong RBS combination </li> |
</ul> | </ul> | ||
</p> | </p> | ||
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<br> | <br> | ||
| − | <h4><u> | + | <h4><u>Flagellin C from <i>Escherichia coli</i> </u></h4> |
| − | <p> | + | <p>Flagellin C (FliC) protein from <i>Escherichia coli</i> strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal such as Platinum, gold...</p> |
| − | <p> We | + | <p> We made a FliC mutant by transduction using phage P1 in a <i> E. Coli W3110 </i> strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110</p> |
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<br> | <br> | ||
