Difference between revisions of "Team:Aix-Marseille/Design"

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<p> Lysine descarboxylase from <i>Streptomyces coelicolor</i> is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.  
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<p> Platinum leaching makes a accumulation of this metal on the road side. By default, it has been prooved that plants are potentiel bioaccumulator of platinum, which is found concentrated on the roots, leaves.. Otherwise, the metal is also carried on the sewage sludge and is actually an issue regarding the recycling potencial of these sludge </p>
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In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.
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We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for <i>E.coli</i> and ordered the synthesis by addition of an inductible promoter.</p>
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<h3 class="titrepage">BBa_K1941008 : FliC <i>E. coli</i> producer</h3>
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<h4><u>Part Composition:</u><h4>
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<p>This part is a composite part composed of 2 Biobricks :
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    <div class="container">
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<ul>
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  <li>BBa_K1951005: flagelln C</li>
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    <div class="col-md-6 left">
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  <li>BBa_K880005: Strong promoter, strong RBS combination </li>
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    <div align="center"><h2 class="title wow bounce in up"><span style="color:#0067B6"><span style="font-family:Armalite Rifle"><u>Mobilisation by a siderophore</div>
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</h2></span></span></u>
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<h3 class="titrepage"> Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al.</h3>
  
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<h4><u>Flagellin C from <i>Escherichia coli</i> </u></h4>
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In the previous article, authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach.  
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<p>Flagellin C (FliC) protein from <i>Escherichia coli</i> strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal such as Platinum, gold...</p>
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<p> We made a FliC mutant by transduction using phage P1 in a <i> E. Coli W3110 </i> strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110</p>
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<h3 class="titrepage"> Pathway of the desferrioxamine B biosynthesis </h3>
  
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"image voie de biosynthese de siderophore"
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Our strategy results for the suderophore mobilisation result in two main steps :
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- Transformation of E.Coli with an inductible plasmide holding the <i><u>des</u></i> cluster
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- Induction and production of the desferrioxamine B
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Then the produced siderophore would be transfered in the recuperation solution containing the platinum. Therefore, the platinum and other metal must be recruit by our purified siderophore. Though those siderophores would be import using a sowing of <i>Streptomyces coelicolor </i>. Then simple centrifugation and combustion will concentrate the solution in metal a first time.
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    <div align="center"><h2 class="title wow bounce in up"><span style="color:#0067B6"><span style="font-family:Armalite Rifle"><u>Biosorption and reduction</div>
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<h4><u>Part Assembly:</u></h4>
 
<h4><u>Part Assembly:</u></h4>

Revision as of 12:27, 12 October 2016