Difference between revisions of "Team:Aix-Marseille/Design"
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"image voie de biosynthese de siderophore" | "image voie de biosynthese de siderophore" | ||
| − | Our strategy | + | Our strategy for the siderophore mobilisation results in two main steps : |
<ul> | <ul> | ||
<li> Transformation of E.Coli with an inductible plasmide holding the <i><u>des</u></i> cluster</li> | <li> Transformation of E.Coli with an inductible plasmide holding the <i><u>des</u></i> cluster</li> | ||
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<li> Induction and production of the desferrioxamine B</li> | <li> Induction and production of the desferrioxamine B</li> | ||
</ul> | </ul> | ||
| − | Then the produced siderophore would be transfered in the recuperation solution containing the platinum. Therefore, the platinum and other metal must be recruit by our purified siderophore. Though those siderophores would be import using a sowing of <i>Streptomyces coelicolor </i>. Then simple centrifugation and combustion will concentrate the solution in metal a first time. | + | |
| + | <p>Then the produced siderophore would be transfered in the recuperation solution containing the platinum. Therefore, the platinum and other metal must be recruit by our purified siderophore. Though those siderophores would be import using a sowing of <i>Streptomyces coelicolor </i>. Then simple centrifugation and combustion will concentrate the solution in metal a first time. </p> | ||
<div class='content_wrapper'> | <div class='content_wrapper'> | ||
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<li> the ambient reducer power reduces ions into nanoparticules </li> | <li> the ambient reducer power reduces ions into nanoparticules </li> | ||
</ul> | </ul> | ||
| − | Deplanche & al.,2008 or Capeness & al.,2015 have shown that flagellar are high adsorber of platinum. Also, Urartu Ozgur & al., 2011 investigated that peptides can bind precious metal like gold, silver, platinum specifically. | + | <p>Deplanche & al.,2008 or Capeness & al.,2015 have shown that flagellar are high adsorber of platinum. Also, Urartu Ozgur & al., 2011 investigated that peptides can bind precious metal like gold, silver, platinum specifically. |
| − | [[File:T--Aix-Marseille--Flagel_agregat.jpeg.jpg]] | + | The aim of our work is to create a Biobrick containing the flagellar sequence with a transcritpion control under strong promotor. Then on the part of the flagellin faced to the extarnal medium when the polymere is formed, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorbsion specificity and yield. |
| + | [[File:T--Aix-Marseille--Flagel_agregat.jpeg.jpg]]</p> | ||
