Difference between revisions of "Team:Aix-Marseille/Design"

(Biosorption and reduction using flagellin and peptides)
(Biosorption and reduction using flagellin and peptides)
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All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinium based on ''E. coli'' and ''Desulfovibrio vulgaris'' flagellum and synthetic peptides sequences. To this end, we analyze the flagellin sequence and structural proprieties of the external part of the flagel. Then, on the part of the flagellin faced to the extarnal medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorbsion specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production.
 
All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinium based on ''E. coli'' and ''Desulfovibrio vulgaris'' flagellum and synthetic peptides sequences. To this end, we analyze the flagellin sequence and structural proprieties of the external part of the flagel. Then, on the part of the flagellin faced to the extarnal medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorbsion specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production.
 
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[[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]]
 
Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions of the medium and reduce them into oxydised nanoparticules usable in industry. A simple centrifugation of the flagellin binding to the platinum allow to concentrate the metal a second time.
 
Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions of the medium and reduce them into oxydised nanoparticules usable in industry. A simple centrifugation of the flagellin binding to the platinum allow to concentrate the metal a second time.
  
 
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Revision as of 20:27, 12 October 2016