(→Monday, August 8th) |
(→Tuesday, August 9th) |
||
Line 537: | Line 537: | ||
===== Tuesday, August 9th ===== | ===== Tuesday, August 9th ===== | ||
− | * Colony PCR from transformation of the 08.08.2016 (size waited : 3000pb), clone1 has been sent to the sequencing | + | * Colony PCR from transformation of the 08.08.2016 (size waited : 3000pb), clone1 has been sent to the sequencing. |
* Plasmid purification of Bba_K1951008 (126.52ng/µL), Bba_K1951002 (102.18ng/µL), Bba_K1951007 (8,33ng/µL) ([https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.233_:_Plasmid_DNA_purification Protocol#4]). | * Plasmid purification of Bba_K1951008 (126.52ng/µL), Bba_K1951002 (102.18ng/µL), Bba_K1951007 (8,33ng/µL) ([https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.233_:_Plasmid_DNA_purification Protocol#4]). | ||
* From the IDT sequence and gel extract of the 08.08.2016, PCR using Q5 master Mix with 2 different programs on DesB and DesD | * From the IDT sequence and gel extract of the 08.08.2016, PCR using Q5 master Mix with 2 different programs on DesB and DesD | ||
− | - 98°C 30s, (98°C 10s, 60°C 30s, 72°C 1min)*30, 72°C 2min | + | - 98°C 30s, (98°C 10s, 60°C 30s, 72°C 1min)*30, 72°C 2min. |
− | - 98°C 30s, (98°C 10s, 72°C 1min)*30, 72°C 2min | + | - 98°C 30s, (98°C 10s, 72°C 1min)*30, 72°C 2min. |
</div> | </div> |