Difference between revisions of "Team:Aix-Marseille/Notebook WIP"
| Line 42: | Line 42: | ||
<li>pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup</li> | <li>pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup</li> | ||
<li>FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3</li> | <li>FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3</li> | ||
| − | <li>E. Coli and Desulfo FliC ligation in linearized pSB1C3<li> | + | <li>E. Coli and Desulfo FliC ligation in linearized pSB1C3</li> |
<li>Transformation of pSB1C3 RFP in Tg1</li> | <li>Transformation of pSB1C3 RFP in Tg1</li> | ||
Revision as of 12:27, 5 July 2016
Monday, June 13th
- Preparation of heat competent DH5α cells
Tuesday, June 14th
- Transformation of DH5α cells with test plasmid
Wednesday, June 15th
- Preparation of heat competent Tg1 cells
- Transformation of Tg1 cells with test plasmid
Thursday, June 16th
- Transformation of DH5α cells with pSB1C3 + RFP/li>
- Transformation with K880005, K823017, I0500, K1404006, B0034
Friday, June 17th
- Miniprep K880005, K823017, I0500, K1404006, B0034
- Miniprep pSB1C3 + RFP
Monday, June 20th
- pSB1C3 digestion from 17/06 miniprep
- pSB1C3 digestion gel migration : NO PHOTO
- Biobricks minipreps gel migration : OK PHOTO
- Transformation Tg1 with RFP+pSB1C3
Tuesday, June 21th
- pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup
- FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3
- E. Coli and Desulfo FliC ligation in linearized pSB1C3
- Transformation of pSB1C3 RFP in Tg1
- Transformation in DH5α of :
- FliC coli/desulfo in pSB1C3
- Linearized pSB1K3
- Linearized pSB1A3
- Linearized pSB1T3
- Linearized pSB1C3
- Miniprep B0034
- Transformation in DH5α of interlab studies plasmids
