Difference between revisions of "Team:Aix-Marseille/Notebook WIP"

Line 42: Line 42:
 
<li>pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup</li>
 
<li>pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup</li>
 
<li>FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3</li>
 
<li>FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3</li>
<li>E. Coli and Desulfo FliC ligation in linearized pSB1C3<li>
+
<li>E. Coli and Desulfo FliC ligation in linearized pSB1C3</li>
 
<li>Transformation of pSB1C3 RFP in Tg1</li>
 
<li>Transformation of pSB1C3 RFP in Tg1</li>
  

Revision as of 12:27, 5 July 2016

Monday, June 13th
  • Preparation of heat competent DH5α cells
Tuesday, June 14th
  • Transformation of DH5α cells with test plasmid
Wednesday, June 15th
  • Preparation of heat competent Tg1 cells
  • Transformation of Tg1 cells with test plasmid
Thursday, June 16th
  • Transformation of DH5α cells with pSB1C3 + RFP/li>
  • Transformation with K880005, K823017, I0500, K1404006, B0034
Friday, June 17th
  • Miniprep K880005, K823017, I0500, K1404006, B0034
  • Miniprep pSB1C3 + RFP
Monday, June 20th
  • pSB1C3 digestion from 17/06 miniprep
  • pSB1C3 digestion gel migration : NO PHOTO
  • Biobricks minipreps gel migration : OK PHOTO
  • Transformation Tg1 with RFP+pSB1C3
Tuesday, June 21th
  • pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup
  • FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3
  • E. Coli and Desulfo FliC ligation in linearized pSB1C3
  • Transformation of pSB1C3 RFP in Tg1
  • Wednesday, June 22th
    • Transformation in DH5α of :
      • FliC coli/desulfo in pSB1C3
      • Linearized pSB1K3
      • Linearized pSB1A3
      • Linearized pSB1T3
      • Linearized pSB1C3
    • Miniprep B0034
    • Transformation in DH5α of interlab studies plasmids