Difference between revisions of "Team:UNebraska-Lincoln/design"

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<p><span class="image left"><img src="https://static.igem.org/mediawiki/2016/5/52/T--UNebraska-Lincoln--greengear.png
 
<p><span class="image left"><img src="https://static.igem.org/mediawiki/2016/5/52/T--UNebraska-Lincoln--greengear.png
 
" alt="" style="width:100px;height:auto;" transform:scale(1.0) /></span></p><font color="silver"><h2>Further Reductases</h2>
 
" alt="" style="width:100px;height:auto;" transform:scale(1.0) /></span></p><font color="silver"><h2>Further Reductases</h2>
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Future work could be done to complete and assemble the reductases necessary to reduce nitrate to nitrogen gas. Similar work has been done by the BYU Provo 2014 iGEM team as well as the 2013 DTU-Denmark team. By combining these efforts with our own, an ecologically friendly organism could be created to completely remove excess nitrate from waterways.</font>
 
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Revision as of 17:45, 18 October 2016

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Project Design

Nitrate Reductase (napDABC)

A coding sequence for the synthetic operon that encodes for four of the proteins that make up the Periplasmic Nitrate Reductase. The operon was assembled from pieces that were PCRed from E. coli and other sequences were synthesized by IDT in order to remove illegal restriction enzyme sites. Due to time constraints complete characterization couldn’t be accomplished.

Nitrate-Sensitive Kill Switch (PyeaR-SerA)

 A composite part designed to act as a nitrate-sensitive kill-switch. The yeaR promoter was originally used by the Edinburgh 2009 iGEM team and was shown to be nitrate sensitive. By combining it with the serA sequence it’s capable of acting as a kill-switch that complements a ΔserA strain of E. coli under high nitrate concentration conditions, but causes the E. coli to die under low nitrate conditions.

Further Reductases

Future work could be done to complete and assemble the reductases necessary to reduce nitrate to nitrogen gas. Similar work has been done by the BYU Provo 2014 iGEM team as well as the 2013 DTU-Denmark team. By combining these efforts with our own, an ecologically friendly organism could be created to completely remove excess nitrate from waterways.