Team:Aix-Marseille/Composite Part 

style="color:#8E3B8C">New biobricks we designed</div>

</h2></span>

We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:
  • -Cytochrome C Synechocystis sp (BBa_K1601001), a cyanobacteria that suggests a cytochrome c

  • -Cytochrome C of S.oneidensis (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.

  • -Heme A from E.coli K12 (BBa_K1601002)that enables the heme overproduction

  • -Yeast signal CXXCH from Synechocystis sp responsible for the S.oneidensis CytC translocation into the periplasm using TAT system (BBa_S05319)

  • -Yeast signal CXXCH from Synechocystis sp responsible for the Synechocystis sp CytC translocation into the periplasm using TAT system (BBa_S05320)

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<section style="padding:30px 0px 50px;" class="arrow_box" id="team">

Linkers we designed</div>

</h2>

<p class="space20">
We designed some linker to facilitate the meeting of the laccase and the cytochrom C </p>
<p class="space20">
  • Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC S.oneidensis (BBa_K1601008)

  • Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC P.denitrificans (BBa_K1601011)

  • Rigid and structured linker designed to link (or connect) T.Thermophilus and CytC Synechocystis sp(BBa_K1601013)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC P.denitrificans (BBa_K1601009)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC Synechocystis sp (BBa_K1601010)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC S.oneidensis(BBa_K1601012)
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<section style="padding:30px 0px 50px;" class="arrow_box" id="team">

<h2 class="title wow bounce in up">New biobricks using existing ones</div>

</h2>

<p class="space20">
We use existing biobrick to create new biobricks we needed:
  • -Yeast signal CXXCH from Synechocystis sp responsible for the S. oneidensis CytC translocation into the periplasm using TAT system
  • -T7 promoter with RBS and Laccase E.coli (Bba_K863006) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601003)
  • -T7 promoter with RBS and Laccase T.Thermophilus (Bba_K863011) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601004)
  • -Cytochrome C Heme Lyase (CCHL)(BBa_K1601005)
  • -Yeast signal CXXCH (BBa_K1601006)
  • -Cytochrome C P.Denitrificans (BBa_K1601007)
  • -Cytochrome C Synechocystis sp - His Tag (BBa_S05314)
  • -Cytochrome C S.oneidensis - HisTag (BBa_S05315)

</div></p> 







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   </section>

<section style="padding:30px 0px 50px;" class="arrow_box" id="team">

<h2 class="title wow bounce in up">New biobricks using existing ones</div>

</h2>

<p class="space20">
We use existing biobrick to create new biobricks we needed:
  • Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)
  • Cytochrome C P.Denitrificans - His Tag (BBa_S05317)
  • Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)
  • T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)

</div></p> 






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