Difference between revisions of "Team:Aix-Marseille/Composite Part"
(→BBa_K1941011 : Desferrioxamine B producer pathway Streptomyces coelicolor producer) |
(→BBa_K1951008 : FliC E. coli producer) |
||
| Line 24: | Line 24: | ||
[[File:T--Aix-Marseille--prom.jpeg|200px|right]] | [[File:T--Aix-Marseille--prom.jpeg|200px|right]] | ||
This part is a composite part composed of 2 Biobricks : | This part is a composite part composed of 2 Biobricks : | ||
| − | * BBa_K1951005: | + | * [http://parts.igem.org/Part:BBa_K1951005 BBa_K1951005] : Flagellin C |
| − | * BBa_K880005: Strong promoter, strong RBS combination | + | * [http://parts.igem.org/Part:BBa_K880005 BBa_K880005] : Strong promoter, strong RBS combination |
====Flagellin C from ''Escherichia coli''==== | ====Flagellin C from ''Escherichia coli''==== | ||
| − | Flagellin C (FliC) protein from ''Escherichia coli'' strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that | + | Flagellin C (FliC) protein from ''Escherichia coli'' strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that flagellin has the ability to adsorb precious metal such as platinum or gold. |
| − | + | We made a FliC mutant by transduction using phage P1 in a ''E. Coli'' W3110 strain. Then we have complemented the ''fliC'' mutant W3110 with [http://parts.igem.org/Part:BBa_K151008 BBa_K151008] and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented ''fliC'' mutant W3110 | |
| − | We made a FliC mutant by transduction using phage P1 in a ''E. Coli | + | |
====Part Assembly:==== | ====Part Assembly:==== | ||
