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| − | ==Which medals | + | ==Which medals do compete for ?== |
===Bronze medal=== | ===Bronze medal=== | ||
To compete for the bronze medal, we have set up the [http://parts.igem.org/Part:BBa_K1951000 BBa_K1951000] simple biobrick. We have designed this biobrick from the sequence of desA of Streptomyces coelicolor A3(2) (complete genome here). It codes for the lysine descarboxylase DesA. It is an enzyme from the lyase family that converts lysine to cadaverine. desA is the first gene from the des operon. This operon is involved in biosynthesis of the Desferrioxamine B, a bacterial siderophore using to bind ferric ion from the environment. As this sequence comes from a gram positive bacterium (i.e., Streptomyces coelicolor A3(2)), we obtimised codon for expression in Escherichia coli by using codon optimization IDT software[1].
To compete for the silver medal, we have set up the [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004] composite biobrick. This biobrick were designed from [http://parts.igem.org/Part:BBa_I0500 I0500] (pARA/araC inducible promoter), [http://parts.igem.org/Part:BBa_B0034 B0034] (Ribosome Binding Site) and our designed biobrick [http://parts.igem.org/Part:BBa_K1951000 K1951000] (Lysine decarboxylase DesA). To test its functionnality, we have complemented the cadA mutant with our biobrick [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004]. Results have shown a recovery of the cadaverine biosynthesis and even beyond the rate obtained with the WT strain. To more information, please visit this [http://parts.igem.org/Part:BBa_K1951004:Experience page].
iGEM 2016 Aix Marseille <groupparts>igem2016 Aix-Marseille</groupparts>
