Difference between revisions of "Team:Aix-Marseille/Medals"

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===Silver medal===
 
===Silver medal===
  
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To compete for the silver medal, we have set up the '''[http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004] functionnal composite biobrick'''. This biobrick were designed from [http://parts.igem.org/Part:BBa_I0500 I0500] (pARA/araC inducible promoter), [http://parts.igem.org/Part:BBa_B0034 B0034] (Ribosome Binding Site) and our designed biobrick  [http://parts.igem.org/Part:BBa_K1951000 K1951000] (Lysine decarboxylase DesA).
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To compete for the silver medal, we have constructed a '''[http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004] functionnal composite biobrick''', that works as expected. This biobrick were designed from [http://parts.igem.org/Part:BBa_I0500 I0500] (pARA/araC inducible promoter), [http://parts.igem.org/Part:BBa_B0034 B0034] (Ribosome Binding Site) and our basic biobrick  [http://parts.igem.org/Part:BBa_K1951000 K1951000] (Lysine decarboxylase DesA).
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To test its functionnality, we have complemented the ''cadA'' mutant with our biobrick [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004]. Results have shown a recovery of the cadaverine biosynthesis and even beyond the rate obtained with the WT strain. To more information, please visit this [http://parts.igem.org/Part:BBa_K1951004:Experience  page].
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To test for function, we have complemented a ''cadA'' mutant with our biobrick [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004], and demonstrated the recovery of cadaverine biosynthesis. Our results show even more production than obtained with the WT strain. For more information, please visit this [http://parts.igem.org/Part:BBa_K1951004:Experience  page].
  
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We also help other iGEM teams with their project by reading a paper or making a model of plasmid loss. All of this can be found [https://2016.igem.org/Team:Aix-Marseille/Collaborations here].
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We also helped other iGEM teams:
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* the Bordeaux team by extracting data  from an article and analyze them.
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* the Toulouse team by making a model of plasmid loss.  
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All of this can be found [https://2016.igem.org/Team:Aix-Marseille/Collaborations here].
  
 
For our human practices, we present the iGEM competition and the use of the synthetic biology in many [https://2016.igem.org/Team:Aix-Marseille/Engagement events].
 
For our human practices, we present the iGEM competition and the use of the synthetic biology in many [https://2016.igem.org/Team:Aix-Marseille/Engagement events].
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===Gold medal===
 
===Gold medal===
  
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To reach the gold medal, we made a big work to integrate our human practices to our project. You can find all this work [https://2016.igem.org/Team:Aix-Marseille/Integrated_Practices right here].
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To reach the gold medal, we worked hard to integrate our human practices to our project.  
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You can find all this work and how it helped our project [https://2016.igem.org/Team:Aix-Marseille/Integrated_Practices right here].
  
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We also improve an existing BioBrick ([parts.igem.org/Part:BBa_K1951008 BBa K1951008]). This BioBrick is an improve of the Glasgow 2014 team's BioBrick ([http://parts.igem.org/Part:BBa_K1463604 BBa K1463604]) Our BioBrick is optimized to have a strong expression in E.coli (thanks to the codon usage), it RBS has no mutation so the flagellin is well expressed and is '''functionnal''' and our swimming test show that the swimming phenotype of the BioBick is improved thanks to our changes. All the functionnal tests and experiments are listed [https://2016.igem.org/Team:Aix-Marseille/Composite_Part#BBa_K1951008_:_FliC_E._coli_producer here].
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We also improved an existing BioBrick, Glasgow 2014 team's BioBrick ([http://parts.igem.org/Part:BBa_K1463604 BBa K1463604]), making our brick ([http://parts.igem.org/Part:BBa_K1951008 BBa K1951008]). This BioBrick is an improvement of the orignial BioBrick in three ways:
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* The codon usage has been optimized to ensure a strong expression in E.coli,  
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* The RBS has no mutation so the flagellin is well expressed,
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* Our swimming test show that the swimming phenotype of the BioBick is improved thanks to our changes.  
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All the functional tests and experiments, show this part is functional, are listed [https://2016.igem.org/Team:Aix-Marseille/Composite_Part#BBa_K1951008_:_FliC_E._coli_producer here].
  
  
 
{{Aix-Marseille/Footer}}
 
{{Aix-Marseille/Footer}}

Latest revision as of 23:21, 19 October 2016