Difference between revisions of "Team:Aix-Marseille/Results"

(Proof of swimming recovery)
(Proof of swimming recovery)
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We have made a biobrick [http://parts.igem.org/Part:BBa_K1951008 BBa_k1951008] ables to produce Flagellin (FliC protein of the flagellum). In the aim to test the flagellin integrity, we made a <i>fliC</i> mutant in a <i>E. coli</i> W3110 strain by transduction using phage P1 ([https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Step2_:_Transduction_P1 protocol]).  
 
We have made a biobrick [http://parts.igem.org/Part:BBa_K1951008 BBa_k1951008] ables to produce Flagellin (FliC protein of the flagellum). In the aim to test the flagellin integrity, we made a <i>fliC</i> mutant in a <i>E. coli</i> W3110 strain by transduction using phage P1 ([https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Step2_:_Transduction_P1 protocol]).  
  
In the figure the deletion mutant (lower left sector) shows no swimming motility as expected and a small white colony. In contrast the wild-type colony (lower right) has a diffuse halo due to swimming cells around the central white colony. Finally the complemented strain, the deletion mutant complemented with our biobrick, (top panel) shows two colonies with intense halos surrounding them. This illustrated clearly that our biobrick can restore motility and is functional.
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In the figure the deletion mutant (lower left sector) shows no swimming motility as expected and a small white colony. In contrast the wild-type colony (lower right) has a diffuse halo due to swimming cells around the central white colony.  
 +
Finally the complemented strain, the deletion mutant complemented with our biobrick, (top panel) shows two colonies with intense halos surrounding them. This illustrated clearly that our biobrick can restore motility and is functional.
 
The intensity of the halo suggests that a greater proportion of the cells are mobile or swimming is in someway better than the wild-type.
 
The intensity of the halo suggests that a greater proportion of the cells are mobile or swimming is in someway better than the wild-type.
  

Revision as of 21:55, 19 October 2016