Difference between revisions of "Team:Aix-Marseille/Safety"

(Safe Project Design)
(Safe Lab Work)
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[[File:T--Aix-Marseille--Safety.jpeg|600px|center|thumb|Our trainer, Chantal Socia, and us]]
 
[[File:T--Aix-Marseille--Safety.jpeg|600px|center|thumb|Our trainer, Chantal Socia, and us]]
  
Before the beginning of the competition, all of the team members followed a well-being and safety formation with Chantal Socia, a dedicated member of the Laboratoire d'Ingénierie des Systèmes Macromoléculaires [http://lism.cnrs-mrs.fr/ (LISM)].  
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Before the beginning of the competition, all of the team members participated to a safety training with Chantal Socia, a dedicated member of the Laboratoire d'Ingénierie des Systèmes Macromoléculaires [http://lism.cnrs-mrs.fr/ (LISM)].  
Every day, each member used a waterless hand cleaner before and after experiments. Benches were disinfected with bleach and one bench was dedicated for BET visualization procedures. Lab coat was used every time, gloves were used for molecular biology (e.g., [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.234_:_Plasmid_DNA_purification Plasmid DNA Purification], [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#PCR PCR]) and biochemestry (e.g., [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Electrophoresis Electrophoresis]), glasses and mask were used for procedure such as [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.239_:_Cadaverin_HPLC_analysis prelimary HPLC protocol] (High Performance Liquid Chromatography) or [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.237_:_PCR_clean-up_and_gel_extraction gel extraction]. Biowastes and chemical wastes were sorted in the respect of the lab rules. Laboratory hood were only used for [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Derivatization_reaction HPLC derivatization reaction step].
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Benches were regularly disinfected with bleach and one bench was dedicated for BET visualization procedures. We always wore lab coats and gloves for molecular biology (e.g., [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.234_:_Plasmid_DNA_purification Plasmid DNA Purification], [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#PCR PCR]) and biochemestry (e.g., [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Electrophoresis Electrophoresis]), glasses and a mask were used for experiments such as [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.239_:_Cadaverin_HPLC_analysis prelimary HPLC protocol] (High Performance Liquid Chromatography) or [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.237_:_PCR_clean-up_and_gel_extraction gel extraction]. Biowastes and chemical wastes were sorted according to lab rules. Laboratory hoods were only used for [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Derivatization_reaction HPLC derivatization reaction step].
  
 
===Safe Shipment===
 
===Safe Shipment===
  
No safety problems have been encoutered in sending DNA parts to the Registry.
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No safety problems have been encountered in sending DNA parts to the Registry.
  
 
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Revision as of 19:11, 19 October 2016