Team:NRP-UEA-Norwich/Parts
Parts
All BioBricks consist of coding sequences for HydABC and HyaABC subunits for the FeFe & NiFe hydrogenase enzymes. These were ligated into the pSB1C3 iGEM vector. All BioBricks samples have been tested for accuracy by DNA sequencing. All gene parts in these BioBricks originated from the organism Shewanella oneidensis MR-1 and were submitted to the iGEM registry on the 19th of October.
| Name in Registry | Backbone | Gene expressed | Function | Length (bp) |
|---|---|---|---|---|
| [http://parts.igem.org/Part:BBa_K1957001 BBa_K1957001] | pSB1C3 | Hyd C (Fdh) | FeFe hydrogenase outer periplasmic subunit | 705 |
| [http://parts.igem.org/Part:BBa_K1957002 BBa_K1957002] | pSB1C3 | Hyd B (SII C-terminus) | FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus | 374 |
| [http://parts.igem.org/Part:BBa_K1957003 BBa_K1957003] | pSB1C3 | Hyd B (SII N-terminus) | FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus | 374 |
| [http://parts.igem.org/Part:BBa_K1957004 BBa_K1957004] | pSB1C3 | Hyd A | FeFe hydrogenase membrane bound catalytic subunit | 1266 |
| [http://parts.igem.org/Part:BBa_K1957005 BBa_K1957005] | pSB1C3 | Hya A | NiFe hydrogenase central subunit | 1156 |
| [http://parts.igem.org/Part:BBa_K1957006 BBa_K1957006] | pSB1C3 | Hya C | NiFe hydrogenase outer periplasmic subunit | 695 |
| [http://parts.igem.org/Part:BBa_K1957007 BBa_K1957007] | pSB1C3 | Hya B (SII C-terminus) | NiFe hydrogenase membrane bound catalytic subunit | 1771 |
| [http://parts.igem.org/Part:BBa_K1957008 BBa_K1957008] | pSB1C3 | Hya B (SII N-terminus) | NiFe hydrogenase membrane bound catalytic subunit | 1771 |
| [http://parts.igem.org/Part:BBa_K1957009 BBa_K1957009] | pSB1C3 | HydABC, FeFe Hydrogenase gene cluster (SII N-terminus) | FeFe hydrogenase gene cluster. Strep II tag incorporated onto the N-terminus | 2291 |
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