Difference between revisions of "Team:UNebraska-Lincoln"

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<html><style>
 
  
/********************************* DEFAULT WIKI SETTINGS  ********************************/
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Solid State by HTML5 UP
 
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html, body, div, span, applet, object, iframe,h1, h2, h3, h4, h5, h6, p, blockquote, pre,a, abbr, acronym, address, big, cite, code,del, dfn, em, font, img, ins, kbd, q, s, samp,small, strike, strong, sub, sup, tt, var,b, u, i, center,dl, dt, dd, ol, ul, li,fieldset, form, label, legend,table, caption, tbody, tfoot, thead, tr, th, td {margin: 0;padding: 0;border: 0;outline: 0;font-size: 100%;vertical-align: baseline;background: transparent;}body {line-height: 1;}ol, ul {list-style: none;}blockquote, q {quotes: none;}blockquote:before, blockquote:after,q:before, q:after {content: '';content: none;} /* remember to define focus styles! */ :focus {outline: 0;} /* remember to highlight inserts somehow! */ins {text-decoration: none;}del {text-decoration: line-through;} /* tables still need 'cellspacing="0"' in the markup */ table {border-collapse: collapse;border-spacing: 0;}
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width:auto;
 
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size: 75%;
 
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<link rel="shortcut icon" href="../favicon.ico" type="image/x-icon"/>  
 
<link rel="shortcut icon" href="../favicon.ico" type="image/x-icon"/>  
 
<link rel="stylesheet" href="https://2016.igem.org/Team:UNebraska-Lincoln/css/NavigationMenu?action=raw&amp;ctype=text/css" type="text/css" media="screen"/>
 
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<span class="sdt_descr"></span>
 
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                             <div class="sdt_box">
 
                                                       
 
 
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
 
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
 
                                                         <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
 
                                                         <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
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                 <div class="sdt_box">
 
                 <div class="sdt_box">
 
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
 
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions">Attributions</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/attributions">Attributions</a>
  
 
</div>
 
</div>
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<section id="banner" >
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<section id="wrapper">
<div class="inner" >
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<header >
<h2 style="font-size:50px, padding-top: 10px">Knockout Nitrate</h2>
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<div class="inner" >
<p><font color="Gainsboro">A synthetic biology project by the <a href="http://igem.unl.edu">University of Nebraska-Lincoln iGEM Team</a></font></p>
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</div>
</div>
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</header>
</section>
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<!-- One -->
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<!-- Content -->
<section id="one" class="wrapper spotlight style1">
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<div class="wrapper">
<div class="inner" >
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<div class="inner">
<a class="image"><img src="https://static.igem.org/mediawiki/2016/9/90/T--UNebraska-Lincoln--iGEMAstock2.jpg" alt="" /></a>
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<style>.image{
<div class="content">
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  position: relative;
<h2 class="major" style="font-size:50px">Overview</h2>
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}
<p align="center"><font color="Gainsboro">The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.  
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.image a{
<p>As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!
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  display: block;     
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  position: absolute;
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<section>
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<h2 class="major">Collaboration</h2>
 +
<p><font color="silver">The emphasis that iGEM places on collaboration allowed us to improve our project and connect with other young scientists. We appreciated the opportunities this provided us and understand that it is a reflection of how the scientific process works in the real world. Over the course of the summer, we collaborated with four different teams: Missouri S&T, WashU/Penn State, The University of Virginia, and the University of Georgia. As this was our first year as an iGEM team, we also received assistance from the WashU/Penn State team, who quickly became our good friends and mentor team. </font></p>
 +
<p><font color="silver"> In July, we attended the Heartland Synthetic Biology Meetup, hosted by Missouri S&T. We helped Missouri S&T and Washington University host a public forum discussion on synthetic biology and host an educational event for children. </font></p>
 +
<p><font color="silver">Our collaboration with the Virginia iGEM team consisted of us assisting them with a survey about biocontainment. This survey will be used to create a pamphlet for future iGEM teams that provides them with information about biocontainment and help make informed decisions in order to improve their practices. Improved biocontainment also serves the dual purpose of inspiring more trust from the public. The team created a badge that is now proudly displayed at the bottom of this webpage.</font></p>
 +
<p><font color="silver">Finally, we helped to characterize the expression of an mCherry gene from the University of Georgia iGEM team. They sent us cells that had already been prepared to express the gene and we used our plate reader to take measurements of the fluorescence of their cells. This was all part of a larger multi-team collaboration effort meant to gather measurements from multiple sources in order to standardize their results.</font></p>
 +
<p><font color="silver">Additionally, the Washington University St. Louis / Penn State iGEM Team  provided us with water samples. We plan to use these samples to test base nitrate levels and to potentially simulate a real-world environment for the testing of our system. They also provided us numerous contacts for potential sponsorship and mentored us throughout the summer. Our teams had similar project backgrounds, and so we were able to discuss our results and exchange ideas.</font></p>
 +
<div id="vgemcollaboration">
 +
<a href="https://2016.igem.org/Team:Virginia/Collaborations" target="_blank"></a>
 
</div>
 
</div>
</div>
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</section>
</section>
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<!-- Two -->
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<!-- Footer -->
<section id="three" class="wrapper alt spotlight style2">
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<section id="footer">
<div class="inner" style=''width:70%;">
+
<div class="inner">
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board" class="image"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--UNebraska-Lincoln--iGEMAstock4.jpg" alt="" /></a>
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<h2 class="major">Want to see more outreach?</h2>
<div class="content">
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench"><img src="https://static.igem.org/mediawiki/2016/a/ac/T--UNebraska-Lincoln--backtothemeetingroom.png" style="width:60%;height:auto;" transform:scale(0.2)"></a></center>
<h2 class="major" style="font-size:50px">The Drawing Board</h2>
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<p><font color=Gainsboro>The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">Check out the drawing board!</a></h2>
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</div>
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</div>
</div>
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</section>
</section>
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<!-- Three -->
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</div>
<section id="three" class="wrapper spotlight style3">
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<div class="inner"style=''width:60%;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory" class="image"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--UNebraska-Lincoln--iGEMAstock3.jpg" alt="" /></a>
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<div class="content">
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<h2 class="major" style="font-size:50px">The Laboratory</h2>
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<p><font color=Gainsboro>Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">Check out the Lab Bench!</a></h2>
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</div>
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</div>
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</section>
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<!-- Two -->
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<section id="three" class="wrapper alt spotlight style2">
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<div class="inner" style=''width:70%;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench" class="image"><img src="https://static.igem.org/mediawiki/2016/e/e9/T--UNebraska-Lincoln--iGEMAstock5.jpg" alt="" /></a>
+
<div class="content">
+
<h2 class="major" style="font-size:50px">Beyond the Bench</h2>
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<p><font color=Gainsboro>Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!</font></p>
+
<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">Check out our Meeting Room!</a></h2>
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</div>
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</div>
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</section>
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<!-- Four -->
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<section id="four" class="wrapper alt style1">
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<div class="inner" style=''width:70%;">
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<h2 class="major" style="font-size:50px">Important:</h2>
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<p>Cras mattis ante fermentum, malesuada neque vitae, eleifend erat. Phasellus non pulvinar erat. Fusce tincidunt, nisl eget mattis egestas, purus ipsum consequat orci, sit amet lobortis lorem lacus in tellus. Sed ac elementum arcu. Quisque placerat auctor laoreet.</p>
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<section class="features">
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="image"><img src="https://static.igem.org/mediawiki/2016/b/bd/T--UNebraska-Lincoln--sponsors.png" alt="" /></a>
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<h3 class="major">Our Sponsors</h3>
+
<p><font color="silver">Our project and iGEM experience would not have been possible without our sponsors!</font></p>
+
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="special">Learn more</a>
+
</article>
+
<article>
+
<a href="#" class="image"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--UNebraska-Lincoln--thanks3.png" alt="" /></a>
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<h3 class="major">Attributions</h3>
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<p><font color="silver">Attribution of all external assistance, art, and web design.</font></p>
+
<a href="#" class="special">Learn more</a>
+
</article>
+
<article>
+
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="image"><img src="https://static.igem.org/mediawiki/2016/4/4b/T--UNebraska-Lincoln--iGEMAstock6.jpg" alt="" /></a>
+
<h3 class="major">Meet the Team!</h3>
+
<p><font color="silver">Our highly diverse of team of six undergraduates spans six majors and two countries.</font></p>
+
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="special">Learn more</a>
+
</article>
+
<article>
+
<a href="#" class="image"><img src="https://static.igem.org/mediawiki/2016/a/a6/T--UNebraska-Lincoln--SafetyStock.jpg" alt="" /></a>
+
<h3 class="major">Safety</h3>
+
<p><font color="silver">Lorem ipsum dolor sit amet, consectetur adipiscing vehicula id nulla dignissim dapibus ultrices.</font></p>
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<a href="#" class="special">Learn more</a>
+
</article>
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<li><a href="#" class="button">Browse All</a></li>
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<h2 class="major">Get in touch</h2>
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<p>Cras mattis ante fermentum, malesuada neque vitae, eleifend erat. Phasellus non pulvinar erat. Fusce tincidunt, nisl eget mattis egestas, purus ipsum consequat orci, sit amet lobortis lorem lacus in tellus. Sed ac elementum arcu. Quisque placerat auctor laoreet.</p>
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<p class="text-muted">Directory: <a href="https://2016.igem.org/Team:UNebraska-Lincoln/libraries">/Libraries </a>I <a href="https://2016.igem.org/Team:UNebraska-Lincoln/css">/css</a></p>
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Revision as of 05:06, 19 October 2016

<!DOCTYPE HTML> Elements - Solid State by HTML5 UP

Collaboration

The emphasis that iGEM places on collaboration allowed us to improve our project and connect with other young scientists. We appreciated the opportunities this provided us and understand that it is a reflection of how the scientific process works in the real world. Over the course of the summer, we collaborated with four different teams: Missouri S&T, WashU/Penn State, The University of Virginia, and the University of Georgia. As this was our first year as an iGEM team, we also received assistance from the WashU/Penn State team, who quickly became our good friends and mentor team.

In July, we attended the Heartland Synthetic Biology Meetup, hosted by Missouri S&T. We helped Missouri S&T and Washington University host a public forum discussion on synthetic biology and host an educational event for children.

Our collaboration with the Virginia iGEM team consisted of us assisting them with a survey about biocontainment. This survey will be used to create a pamphlet for future iGEM teams that provides them with information about biocontainment and help make informed decisions in order to improve their practices. Improved biocontainment also serves the dual purpose of inspiring more trust from the public. The team created a badge that is now proudly displayed at the bottom of this webpage.

Finally, we helped to characterize the expression of an mCherry gene from the University of Georgia iGEM team. They sent us cells that had already been prepared to express the gene and we used our plate reader to take measurements of the fluorescence of their cells. This was all part of a larger multi-team collaboration effort meant to gather measurements from multiple sources in order to standardize their results.

Additionally, the Washington University St. Louis / Penn State iGEM Team provided us with water samples. We plan to use these samples to test base nitrate levels and to potentially simulate a real-world environment for the testing of our system. They also provided us numerous contacts for potential sponsorship and mentored us throughout the summer. Our teams had similar project backgrounds, and so we were able to discuss our results and exchange ideas.