Difference between revisions of "Team:UNebraska-Lincoln"

 
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Solid State by HTML5 UP
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
 
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
 
                                                         <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
 
                                                         <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions">
 
<img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/>
 
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
 
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/attributions">Attributions</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions">Attributions</a>
  
 
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<h2 style=" height: 90px; font-size:45px, padding-bottom: 30px">Knockout Nitrate</h2>
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<p><font color="Gainsboro">A synthetic biology project by the <a href="http://igem.unl.edu">University of Nebraska-Lincoln iGEM Team</a></font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Description" class="image"><img src="https://static.igem.org/mediawiki/2016/9/90/T--UNebraska-Lincoln--iGEMAstock2.jpg" alt="" /></a>
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<h2 class="major" style="font-size:50px">Overview</h2>
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<p align="center"><font color="Gainsboro">The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.  
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<p>As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!
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<h2 class="major">Collaboration</h2>
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<p><font color="silver">The emphasis that iGEM places on collaboration allowed us to improve our project and connect with other young scientists. We appreciated the opportunities this provided us and understand that it is a reflection of how the scientific process works in the real world. Over the course of the summer, we collaborated with four different teams: Missouri S&T, WashU/Penn State, The University of Virginia, and the University of Georgia. As this was our first year as an iGEM team, we also received assistance from the WashU/Penn State team, who quickly became our good friends and mentor team. </font></p>
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<p><font color="silver"> In July, we attended the Heartland Synthetic Biology Meetup, hosted by Missouri S&T. We helped Missouri S&T and Washington University host a public forum discussion on synthetic biology and host an educational event for children. </font></p>
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<p><font color="silver">Our collaboration with the Virginia iGEM team consisted of us assisting them with a survey about biocontainment. This survey will be used to create a pamphlet for future iGEM teams that provides them with information about biocontainment and help make informed decisions in order to improve their practices. Improved biocontainment also serves the dual purpose of inspiring more trust from the public. The team created a badge that is now proudly displayed at the bottom of this webpage.</font></p>
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<p><font color="silver">Finally, we helped to characterize the expression of an mCherry gene from the University of Georgia iGEM team. They sent us cells that had already been prepared to express the gene and we used our plate reader to take measurements of the fluorescence of their cells. This was all part of a larger multi-team collaboration effort meant to gather measurements from multiple sources in order to standardize their results.</font></p>
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<p><font color="silver">Additionally, the Washington University St. Louis / Penn State iGEM Team  provided us with water samples. We plan to use these samples to test base nitrate levels and to potentially simulate a real-world environment for the testing of our system. They also provided us numerous contacts for potential sponsorship and mentored us throughout the summer. Our teams had similar project backgrounds, and so we were able to discuss our results and exchange ideas.</font></p>
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<h2 class="major">Want to see more outreach?</h2>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board" class="image"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--UNebraska-Lincoln--iGEMAstock4.jpg" alt="" /></a>
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench"><img src="https://static.igem.org/mediawiki/2016/a/ac/T--UNebraska-Lincoln--backtothemeetingroom.png" style="width:60%;height:auto;" transform:scale(0.2)"></a></center>
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<h2 class="major" style="font-size:50px">The Drawing Board</h2>
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<p><font color=Gainsboro>The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">Check out the drawing board!</a></h2>
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<div class="content">
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<h2 class="major" style="font-size:50px">The Laboratory</h2>
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<p><font color=Gainsboro>Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">Check out the Lab Bench!</a></h2>
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<h2 class="major" style="font-size:50px">Beyond the Bench</h2>
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<p><font color=Gainsboro>Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!</font></p>
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<h2><a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">Check out our Meeting Room!</a></h2>
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<h2 class="major" style="font-size:50px">Important:</h2>
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<section class="features">
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="image"><img src="https://static.igem.org/mediawiki/2016/b/bd/T--UNebraska-Lincoln--sponsors.png" alt="" /></a>
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<h3 class="major">Our Sponsors</h3>
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<p><font color="silver">Our project and iGEM experience would not have been possible without our sponsors!</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors" class="special">Learn more</a>
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</article>
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="image"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--UNebraska-Lincoln--thanks3.png" alt="" /></a>
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<h3 class="major">Attributions</h3>
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<p><font color="silver">Attribution of all external assistance, art, and web design.</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Attributions" class="special">Learn more</a>
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</article>
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="image"><img src="https://static.igem.org/mediawiki/2016/4/4b/T--UNebraska-Lincoln--iGEMAstock6.jpg" alt="" /></a>
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<h3 class="major">Meet the Team!</h3>
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<p><font color="silver">Our highly diverse of team of six undergraduates spans six majors and two countries.</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Team" class="special">Learn more</a>
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</article>
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<article>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="image"><img src="https://static.igem.org/mediawiki/2016/a/a6/T--UNebraska-Lincoln--SafetyStock.jpg" alt="" /></a>
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<h3 class="major">Safety</h3>
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<p><font color="silver">See the steps we took to ensure our own safety and the safety of our project.</font></p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/Safety" class="special">Learn more</a>
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<p class="text-muted">Directory: <a href="https://2016.igem.org/Team:UNebraska-Lincoln/libraries">/Libraries </a>I <a href="https://2016.igem.org/Team:UNebraska-Lincoln/css">/css</a></p>
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Latest revision as of 02:44, 20 October 2016

Solid State by HTML5 UP

Overview

The Nitrogen Cycle is a biogeochemical cycle which has become extremely unbalanced. High nitrate levels in waterways cause drastic effects on the marine life and surrounding environment. To combat this, we set out to engineer an organism capable of reducing these nitrate ions. Throughout our experiments and outreach, we expanded the scope of our project. We developed a nitrate sensitive kill switch, as well as developed safety assurance cases in order to gain confidence in the safety of our system.

As you walk through this wiki, you'll navigate through the drawing board, the laboratory bench, as well as our experiences beyond the bench. Enjoy!

The Drawing Board

The background information, computational modeling, and project design that eventually made its way into our laboratory experiments.

Check out the drawing board!

The Laboratory

Our wet lab experiments, lab notebook procedures, and novel application of safety assurance cases.

Check out the Lab Bench!

Beyond the Bench

Our experiences outside of the lab, from public outreach experiences to inter-team collaboration, we had a blast!

Check out our Meeting Room!

Important:

Our Sponsors

Our project and iGEM experience would not have been possible without our sponsors!

Learn more

Attributions

Attribution of all external assistance, art, and web design.

Learn more

Meet the Team!

Our highly diverse of team of six undergraduates spans six majors and two countries.

Learn more

Safety

See the steps we took to ensure our own safety and the safety of our project.

Learn more