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...n earnest! We received three plasmids from AddGene: pKDsgRNA-p15, pKDsgRNA-ack, and pCas9-CR4. These were <b>transformed</b> into our cloning strain, stre ...E. coli genome. To do so, we performed a <b>SLiM PCR</b> on the pKD-sgRNA-ack plasmid. We <b>digested with DpnI</b> the products of these PCRs to remove

<p>The Shubenacadie Wildlife Park (pronounced "Shoo-ben-ack-a-dee") is about a 45 minute drive outside of Halifax, in the village of Sh