Nanoparticle Attachment: Cyborg Method
*The safety information in the table above is based on the SDS for each chemical.
1. Cedarlane. SDS: Poly(allylamine hydrochloride).
Can be used to attach silver nanoparticles synthesized using Turkevich method or cabbage synthesis method. Attachment to yeast or E.coli.
Cells: E.coli DH5[alpha] Risk group: Level 1 (https://2014.igem.org/Safety/Risk_Group_Guide)
Protocol for attaching silver nanoparticles to E.coli:
1. Add 2mL of citrate-capped silver nanoparticles to 10mL of aqueous polyelectrolyte solution (1% PAH)
2. Sonicate for 20 min using the jewelry sonicator set to “high”
3. The nanoparticle solution needs to be stirred for 24h
4. Centrifuge the solution at high (12000rpm) for 2 min
5. Wash 3 times with water
6. Mix 100 μL of E.coli (3.0*107 cells/mL) or yeast cells (2.5*109 cells/mL) with 1mL of 0.45 mg/mL PE-stabilised silver nanoparticles
7. Incubate for 15 min in a low speed shaker
8. Centrifuge at high 2min to separate cells from loose nanoparticles
9. Wash cells with water
Source: Konnova, S.A., Danilushkina, A.A., Fakhrullina, G.I., Akhatova, F.S., Badrutdinov, A.R., and Fakhrullin, R.F. (2015) Silver nanoparticle-coated “cyborg” microorganisms: rapid assembly of polymer-stabilised nanoparticles on microbial cells. RSC Adv., 5(18):13530-13537. Retrieved from http://pubs.rsc.org/en/content/articlehtml/2015/ra/c4ra15857a
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