Team:HSiTAIWAN/Measurement

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　Oh hello there,I am Charlie,a trusty member of the "Herb Tasters" and also the brainiest E. coli in the colony.
　I know all the secrets of Chinese herbs and their magical healing powers.
　If you are up to a challenge,find me at team HSiTW at the jamboree.
　I am the one in a straw hat,showing them pearls.I will be waiting.
　Muhahahahaha

　Hi there! My name is Nu Zhen Chi. This is how I look like.
　　Take a closer look; guess which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(4) the seed
　Name: 女貞子 (Nu Zhen Chi)
　Botanical Name: Ligustrum lucidum Aiton
　I can treat people who are yin deficient, and liver problems that cause dizziness,cataract of the eyes,
lower back pain, premature graying of the hair and tinnitus.

　Hello! My name is Chuan Xiong. This is how I look like.
　Make a guess, which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(1) the root
　Name: 川芎 (Chuan Xiong)
　Botanical Name: Ligusticum chuanxiong Hort
　I help with blood regulation to prevent relevant to blood stasis and non-stop bleeding.I can also strengthen your qi circulation.
　In addition, I relieve you of physical pain, such as headaches, abdominal aches, chest pain, and muscle pain.
　Finally, I free the ladies of menstrual disorders and amenorrhea.

　What’s up? My name is Dang Gui. I can:
(1) stop coughing
(2) regulate mense
(3) reduce internal heat

　Ans.(2) Regulate mense
　Name: 當歸 (Dang Gui)
　Botanical Name: Angelica sinensis (Oliv.) Diels
　I can remove blood stasis and clots, so I am usually used to regulate menses,lubricate intestines to correct constipation, reduce swelling, expel pus.

　　Reference
　臧堃堂 (2005) 中華材輕百科-現代版本草綱目，山岳文化出版社，台北
　Non-Profit Organization Brion Research Institute of Taiwan.
　Chinese Herb Gallery. Jade Institute
　Herbal Glossary. Shen-Nong- Chinese Traditional Medicine
　　Acknowledgement
　Thank you for Non-Profit Organization Brion Resaerch Institute of Taiwan that provide us Chinese herbs and photos.

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  • Measurement

    • Circuit Design
    • Simulation Result
    • 　　 　　

    Measurement

    Circuit Design for Measuring PbrR generation

    PbrR protein is one kind of MerR regulator which activates the PpbrR promoter in the presence of lead ion. In our project, we transformed a plasmid with PbrR generator into E. coli to design a biosensor to detect lead ion in Chinese medicine. In order to measure the generation of PbrR, we designed two types of PbrR genetic circuits and measure the PbR according to the GFP expression downstream:

    1.Autoregulated PbrR circuit(BBa_K1961004): an autoregulated generator of PbrR measured by GFP expression downstream. PbrR and GFP expression is under the control of TetR and promoter PTet (Figure 1(a))

    2.Constitutive PbrR circuit(BBa_K1961005): a constitutive generator of PbrR measured by GFP expression downstream. PbrR and GFP expression is under the constitutive promoter (Pcon, BBa_J23102 ) (Figure 1(b))

    In order to investigate the response of PbrR in these two circuits, we make use of GFP as a reporter in the downstream as a proxy to measure the PbrR produced by two circuits. For autoregulation PbrR circuit, we want to study in detail about the response of PbrR protein with increasing amount of inducer Tc (or aTc). The advantage of utilizing this autoregulation circuit is to get very precisely for the gradual response with the inducer, which implies that the amount PbrR protein will be the function of inducer. For utilizing the constitutive PbrR, we are able to measure the background expression of PbrR in E. Coli without any inducer. This will help us to compare and recognize the generation of PbrR under autoregulation. All the constructs used here, the Hill coefficient will be calculated to disclose the intrinsic property embedding the both of genetic circuits and be a guide for our biosensor project.

    Figure 1(a). The autoregulated PbrR circuit Figure 1(b). The constitutive PbrR circuit

    Simulation Result of Autoregulated PbrR generation

    Figure 2. The Autoregulation circuit and the related rate equations

    Autoregulation circuit

    Figure 3. Autoregulation PbrR generator circuit: the generation of PbrR measured by GFP expression downstream and is under the control of TetR and promoter PTet

    Autoregulation circuit

    Figure 4. Autoregulation PbrR generator circuit: the generation of PbrR measured by GFP expression downstream and is under the control of TetR and promoter PTet

    Fig 5. The parameter relationship for circuits

    Fig 6. Fold relationship for Autoregulatory and Constitutive circuits