Team:HSiTAIWAN/Notebook/CM/Cu

X

　Oh hello there,I am Charlie,a trusty member of the "Herb Tasters" and also the brainiest E. coli in the colony.
　I know all the secrets of Chinese herbs and their magical healing powers.
　If you are up to a challenge,find me at team HSiTW at the jamboree.
　I am the one in a straw hat,showing them pearls.I will be waiting.
　Muhahahahaha

　Hi there! My name is Nu Zhen Chi. This is how I look like.
　　Take a closer look; guess which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(4) the seed
　Name: 女貞子 (Nu Zhen Chi)
　Botanical Name: Ligustrum lucidum Aiton
　I can treat people who are yin deficient, and liver problems that cause dizziness,cataract of the eyes,
lower back pain, premature graying of the hair and tinnitus.

　Hello! My name is Chuan Xiong. This is how I look like.
　Make a guess, which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(1) the root
　Name: 川芎 (Chuan Xiong)
　Botanical Name: Ligusticum chuanxiong Hort
　I help with blood regulation to prevent relevant to blood stasis and non-stop bleeding.I can also strengthen your qi circulation.
　In addition, I relieve you of physical pain, such as headaches, abdominal aches, chest pain, and muscle pain.
　Finally, I free the ladies of menstrual disorders and amenorrhea.

　What’s up? My name is Dang Gui. I can:
(1) stop coughing
(2) regulate mense
(3) reduce internal heat

　Ans.(2) Regulate mense
　Name: 當歸 (Dang Gui)
　Botanical Name: Angelica sinensis (Oliv.) Diels
　I can remove blood stasis and clots, so I am usually used to regulate menses,lubricate intestines to correct constipation, reduce swelling, expel pus.

　　Reference
　臧堃堂 (2005) 中華材輕百科-現代版本草綱目，山岳文化出版社，台北
　Non-Profit Organization Brion Research Institute of Taiwan.
　Chinese Herb Gallery. Jade Institute
　Herbal Glossary. Shen-Nong- Chinese Traditional Medicine
　　Acknowledgement
　Thank you for Non-Profit Organization Brion Resaerch Institute of Taiwan that provide us Chinese herbs and photos.

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  • Functional Array

    • Pb
    • Cu
    • As
    • CYP

  • Chinese Medicine testing

    • Pb
    • Cu
    • As
    • CYP

    • Chinese Medicine Cu

    2016/07/22

    2016/07/22

    2016/08/11

    2016/08/19

    Topic: Fluorescence, Optical Density Testing in 甘草

    Procedure:

    Control= k1555000without Chinese Medicine or Cu2+

    Control + Cu=k1555000with only [Cu2+] =3ppm

    Control + CM=k1555000with only Chinese Medicine

    Positive Control=k608010

    Negative Control=k1106004

    CM+3ppm=k1555000 with Chinese Medicine and [Cu2+] =3ppm

    CM+9ppm=k1555000 with Chinese Medicine and [Cu2+] =9ppm

    CM+27ppm=k1555000 with Chinese Medicine and [Cu2+] =27ppm

    Chinese Medicine concentration level is 3μg/ml

    Results:

    We want to see whether Chinese Medicine would trigger or stifle the fluorescence expression of our E. coli. As in graph1, we can see that there is no difference in fluorescence intensity when no copper ion is present. The control groups range as low as ournegative control which is constantly inhibited. On the other hand, in graph2, Chinese Medicine does not repress the fluorescent expression, conducting a parallel performance with the control group. Also, this graph indicates that there is significant difference with the manipulating factor of copper ion existence. In graph3, we can still see that the fluorescence intensity of E. coli hikes as the [Cu2+] rises, and reaches a splendid performance at 27ppm (the law standards for Taiwan is 20ppm). Moreover, this graph shows that Chinese Medicine would not interfere the bacteria’s positive relation with copper concentration levels. In graph4, we want to point out that at 27ppm concentration, the fluorescence intensity of bacteria is nearly as strong as our positive control which constantly gives off green fluorescence protein. In short, we can assure that our bacteria are practical in this Chinese Medicine, and thus, our project is achievable.

    2016/08/22


    2016/08/24

    2016/08/27

    Topic: Fluorescence Intensity, Optical Density of E. coli in Different Chinese Herb

    Preparation:(20ml of sample)

    Positive Control:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k608010, k608012) +

    0.2ml of (300ppm) Cu2+solution+

    3μl of (0.1g/ml) Herb Extract


    Negative Control:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k1106004) +

    0.2ml of (300ppm) Cu2+solution+

    3μl of (0.1g/ml) Herb Extract


    Control+Cu:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k1555000) +

    0.2ml of (300ppm) Cu2+solution+

    3μl of ddH2O


    Control+CM:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k1555000) +

    0.2ml of ddH2O

    3μl of (0.1g/ml) Herb Extract


    Control:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k1555000) +

    203μl of H2O


    CM+3ppm:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k1555000) +

    0.2ml of (300ppm) Cu2+solution+

    3μl of (0.1g/ml) Herb Extract


    CM+9ppm:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k1555000) +

    0.2ml of (900ppm) Cu2+solution+

    3μl of (0.1g/ml) Herb Extract


    CM+27ppm:

    　19.4ml of SOC+

    0.4ml of (OD=0.5) E. coli (k1555000) +

    0.2ml of (2700ppm) Cu2+solution+

    3μl of (0.1g/ml) Herb Extract

    Conclusion:

    Although we manipulated a few slight changes between data, but we can still acknowledge an abstract of different Chinese Medicine. Aside fromScutellaria, Bupleurum, and Ligusticum, the other 6 Chinese Medicine appears to be auspicious to the growth curve and fluorescence intensity of the E. coli. With our estimation, we guess that our E. coli can endure a bulk proportion of Chinese Herb; as for the few herbs that may stifle the results, we hope to create a data base for people to easily correspond to different herbs and know our product’s detection limit. Fortunately, we stumbled across a paper claiming that Scutellaria absorbs copper ions by its very own nature. Hence, it will perfectly make sense to the decliningresults offluorescence overtime. To cope with this situation, we decide to add chelating agents into our product to prevent the absorption. Further experiments are expected to examine whether the agent will or will not interfere the bacteria, and the efficiency of different material agents. Lastly, we find that copper ions at the concentration of 27ppm (the law standard in Taiwan is 20 ppm) may suppress the growth and survival of bacteria; yet, the remaining bacteria can express a high level of fluorescence intensity, which means they still great potential even in high toxic concentration levels.

    2016/09/29