Team:HSiTAIWAN/Notebook/FA/As

X

　Oh hello there,I am Charlie,a trusty member of the "Herb Tasters" and also the brainiest E. coli in the colony.
　I know all the secrets of Chinese herbs and their magical healing powers.
　If you are up to a challenge,find me at team HSiTW at the jamboree.
　I am the one in a straw hat,showing them pearls.I will be waiting.
　Muhahahahaha

　Hi there! My name is Nu Zhen Chi. This is how I look like.
　　Take a closer look; guess which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(4) the seed
　Name: 女貞子 (Nu Zhen Chi)
　Botanical Name: Ligustrum lucidum Aiton
　I can treat people who are yin deficient, and liver problems that cause dizziness,cataract of the eyes,
lower back pain, premature graying of the hair and tinnitus.

　Hello! My name is Chuan Xiong. This is how I look like.
　Make a guess, which part of me is used as medicine?
(1) the root
(2) the stem
(3) the leaf
(4) the seed

　Ans.(1) the root
　Name: 川芎 (Chuan Xiong)
　Botanical Name: Ligusticum chuanxiong Hort
　I help with blood regulation to prevent relevant to blood stasis and non-stop bleeding.I can also strengthen your qi circulation.
　In addition, I relieve you of physical pain, such as headaches, abdominal aches, chest pain, and muscle pain.
　Finally, I free the ladies of menstrual disorders and amenorrhea.

　What’s up? My name is Dang Gui. I can:
(1) stop coughing
(2) regulate mense
(3) reduce internal heat

　Ans.(2) Regulate mense
　Name: 當歸 (Dang Gui)
　Botanical Name: Angelica sinensis (Oliv.) Diels
　I can remove blood stasis and clots, so I am usually used to regulate menses,lubricate intestines to correct constipation, reduce swelling, expel pus.

　　Reference
　臧堃堂 (2005) 中華材輕百科-現代版本草綱目，山岳文化出版社，台北
　Non-Profit Organization Brion Research Institute of Taiwan.
　Chinese Herb Gallery. Jade Institute
　Herbal Glossary. Shen-Nong- Chinese Traditional Medicine
　　Acknowledgement
　Thank you for Non-Profit Organization Brion Resaerch Institute of Taiwan that provide us Chinese herbs and photos.

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  • Functional Array

    • Pb
    • Cu
    • As
    • CYP

  • Chinese Medicine testing

    • Pb
    • Cu
    • As
    • CYP

    • Functional Array As

    2016/08/18

    Objective:

    Measure the growth rate of E. coli DH5α in arsenic solution

    Material:

    DH5α pcDNA (ctrl) on LB agar plates

    SOC broth

    Amp (1000 X)

    15 ml tube, 50 ml tube

    96 well plate

    Procedure:

    Rpm 120:

    　1.Culture 3 tubes of 2ml E. coli overnight by 15ml centrifuge tubes (2ml SOC, 2ul AMP)

    　2.Put them to the same tube at the next day, then add 6ml of SOC broth(water bath 37°C for 5 min. before use)and 12ul AMP for a 2-fold dilution.

    　3.Take 5ml SOC broth for to a 15ml centrifuge tube for blank

    　4.Aliquot the bacterial broth into six 15ml centrifuge tubes (2ml for each), then culture for 2 hours

    　5.Measure OD 595

    　6.Dilute to OD 0.5. More than 12ml


    Rpm 80:

    　1.100, 50, 30, 10, 1ppm arsenic solutions, use ddH2O in control group

    　2.Dilute the E. coli broth to 50 fold by SOC broth (0.6ml E. coli broth+0.3ml Arsenic solutuin+30ul AMP 29.1ml SOC broth）

    　3.Measure OD at 0 min. measure every 30 min. Blank is needed every time.


    Arsenic solution preparation:

    　100ppm: 104 As solution 0.3ml

    　50ppm: 104 As solution 0.15ml + ddH2O 0.15ml

    　30ppm: 104 As solution 0.09ml + ddH2O 0.21ml

    　10ppm: 103 As solution 0.3ml

    　1ppm: 102 As solution 0.3ml


    Results:

    2016/08/23 ～ 08/24

    Objective:

    Measure the fluorescence intensity in arsenic solution

    Material:

    DH5α arsenic plasmid (ctrl) on LB agar plates

    SOC broth

    Chloramphenicol(1000 X)

    15 ml tube, 50 ml tube

    96 well plate

    Procedure:

    Rpm 120:

    　1.Culture 3 tubes of 2ml E. coli overnight by 15ml centrifuge tubes (2ml SOC, 2ul Chloramphenicol)

    　2.Put them to the same tube at the next day, then add 6ml of SOC broth(water bath 37°C for 5 min. before use)and 12ul Chloramphenicol for a 2-fold dilution.

    　3.Take 5ml SOC broth for to a 15ml centrifuge tube for blank

    　4.Aliquot the bacterial broth into six 15ml centrifuge tubes (2ml for each), then culture for 2 hours

    　5.Measure OD 595

    　6.Dilute to OD 0.5. More than12ml


    Rpm 80:

    　1.100, 50, 30, 10, 1ppm arsenic solutions, use ddH2O in control group

    　2.Dilute the E. coli broth to 50 fold by SOC broth (0.6ml E. coli broth+0.3ml Arsenic solutuin+30ul Chloramphenicol 29.1ml SOC broth）

    　3.Measure it's fluorescence intensity and OD at 0 min. measure every 30 min. Blank is needed every time.


    Arsenic solution preparation:

    　100ppm: 104 As solution 0.3ml

    　50ppm: 104 As solution 0.15ml + ddH2O 0.15ml

    　30ppm: 104 As solution 0.09ml + ddH2O 0.21ml

    　10ppm: 103 As solution 0.3ml

    　1ppm: 102 As solution 0.3ml


    Results:

    2016/10/02

    Objective:

    Measure the growth rate of E. coli DH5α in As, Cu, Pb solution

    Material:

    DH5α pcDNA (ctrl) on LB agar plates

    SOC broth

    Amp (1000 X)

    15 ml tube, 50 ml tube

    96 well plate

    Procedure:

    Rpm 120:

    　1.Culture 3 tubes of 2ml E. coli overnight by 15ml centrifuge tubes (2ml SOC, 2ul AMP)

    　2.Put them to the same tube at the next day, then add 6ml of SOC broth(water bath 37°C for 5 min. before use)and 12ul AMP for a 2-fold dilution.

    　3.Take 5ml SOC broth for to a 15ml centrifuge tube for blank

    　4.Aliquot the bacterial broth into six 15ml centrifuge tubes (2ml for each), then culture for 2 hours

    　5.Measure OD 595

    　6.Dilute to OD 0.5. More than 12ml


    Rpm 80:

    Arsenic solution preparation:

    1. As:0ppm, Cu:0ppm, Pb:0ppm
    2. As:0ppm, Cu:30ppm, Pb:50ppm
    3. As:100ppm, Cu:0ppm, Pb:0ppm
    4. As:100ppm, Cu:30, Pb:0ppm
    5. As:100ppm, Cu:0ppm, Pb:50
    6. As:100ppm, Cu:30ppm, Pb:50ppm

      7. we use ddH2O in control group

      　2.Dilute the E. coli broth to 50 fold by SOC broth (0.6ml E. coli broth+each 0.3ml of solution prepared+30ul AMP 28.5ml SOC broth）

      　3.Measure OD at 0 min. measure every 30 min. Blank is needed every time.