Team:IIT-Madras/Composite Part
Composite Parts
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:
1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001], [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002], [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003], [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004].
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing GFP and RFP proteins. Part ranges from [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015], [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018], [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023].
3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.
4.sgRNA parts: We have constructed sgRNA coding parts for mCherry, CamR and KanR genes. There parts are: [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006], [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007], [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008], [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009], [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010], [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011], [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012], [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013].
Best Composite Part
Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.
