Team:Macquarie Australia/Electro

pbanner

Electrophoresis




Gel Preparation


  1. Mix 1g of agarose powder with 100ml of 1x TAE buffer and heat for 1 minute or until all agarose is dissolved.
  2. Wait until it has cooled (not set), and add 1ul of GelRed into the mixture.
  3. Pour the solution into a cast with an appropriate comb.
  4. Leave to set.



Running the Gel


  1. Mix 1ul of 1kbp DNA ladder with 6ul of loading dye (bromophenol blue) and 4ul of 1 x TAE buffer (total 6ul) and load onto first well.
  2. Mix 5ul of PCR products with 1ul of loading dye and load into wells.
  3. Run gel at 90V products with 1ul of loading dye and load onto wells.
  4. Run gel at 90V for 45 minutes approximately.
  5. Photograph gels under UV light.