Heat Shock Transformation
- Obtain competent cells from -80oC.
- Defrost gently on ice. 100µl is sufficient for 2 transformations.
- Add 1-10µl of plasmid DNA/ ligation mix to each tube. Incubate on ice for 30 min.
- Heat shock in 42oC water bath for 30 seconds, then back on ice for 2 min.
- Add 200µl of SOC media to each tube, and incubate in the 37oC shaker for 1h.
- For each tube of cells, spread 50µl onto one LB plate with appropriate antibiotic, and 100µl onto a second plate, using aseptic technique. Place your plate upside-down in the 37oC incubator.