Team:NYMU-Taipei/Notebook-Lab Book-BBa K118400

BBa K118400

BBa K118400


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9/6

  • BBa_K118400 resuspended from 2016 iGEM Kit plate 4

  • BBa_K118400 transformed into competent cells

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    9/7

  • BBa_K118400 containing culture used in 2 in 1

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    9/8

  • BBa_K118400 extraction extracted from transformed cultures

  • BBa_K118400 amplified with plasmid PCR

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    9/9

  • BBa_K118400 checked with restriction digestion (the gel had faulty matrix)

  • BBa_K118400 checked with restriction digestion (confirmed, successful)

  • BBa_K1184000 use KOD polymerase to amplify the target part KillerRed (try)

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    9/10

  • BBa_K1184000 use KOD polymerase to amplify the target part KillerRed (really amplified)

  • Digested KillerRed fragment and its backbone pBARGPE1 with BamHI and EcoRI for ligation to construct the vector pBAR_KR

  • Digested KillerRed fragment and its backbone pBARGPE1 with BamHI and EcoRI again

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    9/16

  • Extracted plasmid BBa_K118400

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