Team:NYMU-Taipei/Notebook-Lab Book-C120 empty

C120 empty

C120 empty


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7/4

  • Received plasmid C120_empty from University of Texas Southwestern

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    7/13

  • pC120_empty resuspended (sample from University of Texas Southwestern)

  • pC120_empty transformation attempted (failed, no cultures on first plate)

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    7/14

  • pC120_empty transformation

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    7/15

  • pC120_empty extracted from transformed cultures and checked with PstI restriction digestion

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    7/16

  • pC120_empty plasmid stored and transformed cultures cryopreserved

    • 7/28

  • pC120_empty ran restriction digestion for the ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)

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    7/30

  • pC120_empty digestion for the ligation with HindIII and BamHI

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