Team:NYMU-Taipei/Notebook-Lab Book-Lab note
C120_minPgpdA_RFP_TtrpC
pBAR_PgpdA_EL222_TtrpC
pBAR_PMcl1_EL222_TtrpC
pBAR_PMcl1_KR_TtrpC
pBAR_PMcl1_RFP_TtrpC
pGFP_C120_RFP
6/22: Received plasmid pFC330, pFC331, pFC332, pFC333 and pFC334 from Technical University of Denmark (Danish: Danmarks Tekniske Universitet)
6/29: Received plasmid pBARGPE1 From Bioresource Collection and Research Center
6/30: Received plasmids pCAMBIA1201 from Academia Sinica
6/30: Received plasmids pCAMBIA1300 from National Chung Hsing University
6/30: Received plasmid pTiB0542 from Academia Sinica
7/4: Received plasmid pVP-EL222 from University of Texas Southwestern
7/4: Received plasmid C120_empty from University of Texas Southwestern
7/4: Received plasmid ARSEF549 from ARSEF of USA
7/9: BBa_E1010 T-streaked onto agar plates
7/10: BBa_E1010 extracted plasmid and checked with restriction digestion (EocRI and PstI)
7/11: pBARGPE1 resuspended (from cryovial)
7/11: Transformed pBARGPE1 into competent cells
7/12: pBARGPE1 containing cultures used in 2 in 1
7/12: pBARGPE1 extracted from transformed cultures
7/13: pBARGPE1 checked with restriction digestion (EcoRI and SpeI)
7/13: pC120_empty resuspended (sample from University of Texas Southwestern)
7/13: pC120_empty transformation attempted (failed, no cultures on first plate)
7/13: pVP-EL222 resuspended (sample from University of Texas Southwestern)
7/13: pVP-EL222 transformed into competent cells
7/14: pVP-EL222 did 3 in 1
7/14: pVP-EL222 extracted from transformed cultures and checked with restriction digestion (EcoRI and XhoI)
7/14: pC120_empty transformation
7/15: pC120_empty extracted from transformed cultures and checked with PstI restriction digestion
7/15: pVP-EL222 plasmid stored and transformed cultures cryopreserved
7/16: pC120_empty plasmid stored and transformed cultures cryopreserved
7/16: BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)
7/17: BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)
7/18: BBa_E1010 checked with plasmid PCR with newly design primers
7/19: BBa_E1010 checked with plasmid PCR with the addition of Mg2+
7/21: pBARGPE1 plasmid stored and transformed cultures cryopreserved
7/22: Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station
7/22: Transformed plasmid pAN7-1 into competent cell
7/22: plasmid pAN7-1 did 2 in 1(liquid culture & second plate)
7/22: BBa_E1010 checked with Taq polymerase plasmid PCR (successful)
7/22: pAN7-1 transformation
7/22: pAN7-1 did 2 in 1
7/23: pAN7-1 plasmid extraction
7/23: Extracted plasmid pAN7-1
7/23: Transformed pCAMBIA1300 into competent cells
7/24: pCAMBIA1300 containing cultures used in 2 in 1
7/24: Extracted plasmid pCAMBIA1300
7/24: pCAMBIA1300 digestion check
7/24: pAN7-1 amplified with plasmid PCR (primer: minPgpdA)
7/24: pAN7-1 extracted and transformed cultures cryopreserved
7/24: pAN7-1 did plasmid PCR
7/24: plasmid pAN7-1 stored plasmid and cryopreservation bacterical cultures
7/25: BBa_E1010 amplified with KOD polymerase PCR
7/26: pCAMBIA1300 stored plasmid and transformed cultures cryopreserved
7/26: RFP amplified with KOD polymerase PCR
7/26: RFP digested with EcoRI and BamHI
7/26: Amplified minimal PgpdA promoter on pAN7-1 by doing KOD PCR
7/26: pBARGPE1 digested for pBAR_RFP construction
7/27: PgpdA checked with Taq polymerase plasmid PCR
7/28: Digested minimal PgpdA fragment for ligation with pC120_empty backbone to construct vector pC120_minPgpdA
7/28: pC120_empty ran restriction digestion for ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)
7/29: VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)
7/29: pVP-EL222 amplified with plasmid PCR (contaminated NC)
7/29: pBAR_RFP checked with KOD polymerase PCR (contaminated NC)
7/30: pVP-EL222 checked with plasmid PCR with brand new reagents (success, no contamination detected)
7/30: pC120_empty digestion for ligation with HindIII and BamHI
7/30: pBARGPE1 digested (concentration not high enough)
7/31: Transformed C120_minimal PgpdA into competent cells
7/31: C120_minimal PgpdA containing cells used in 2 in 1
7/31: pVP-EL222 amplified with plasmid PCR
7/31: VP-TR trouble shooting
7/31: VP-TR PCR amplification
8/1: pVP-EL222 digested with SpeI and BamHI)
8/1: C120_minimal PgpdA extracted from transformed cells
8/1: pBARGPE1 digested for pBAR_RFP construction
8/1: RFP checked with plasmid PCR
8/1: C120_minimal PgpdA checked with restriction digestion
8/2: C120_minimal PgpdA amplified with plasmid PCR
8/2: pBAR_RFP containing cultures used in 3 in 1
8/3: Extracted plasmid pBAR_RFP
8/3: pBAR_RFP did digestion check (wrong)
8/3: C120_minimal PgpdA digested for ligate with RFP
8/3: C120_minimal PgpdA_RFP transformed into competent cells
8/3: PgpdA checked with KOD polymerase plasmid PCR
8/3: PgpdA amplified with KOD polymerase plasmid PCR
8/3: RFP digested with EcoRI and BamHI for ligation
8/4: PgpdA digested for pBAR_PgpdA construction
8/4: C120_minimal PgpdA_RFP extracted from transformed cells
8/4: C120_minimal PgpdA_RFP amplified with plasmid PCR and checked with restriction digestion
8/4: pBARGPE1 digested for pBAR_EL222_TtrpC construction
8/5: pBARGPE1 digested for pBAR_PgpdA construction
8/5: pBAR_PgpdA transformed into competent cells
8/5: Transformed pBAR_RFP into competent cells
8/6: pBAR_RFP did 2 in 1
8/6: pBAR_PgpdA containing cultures used in 2 in 1
8/6: C120_minimal PgpdA_RFP plasmid stored and transformed cultures cryopreserved
8/7: pBAR_PgpdA extracted and checked with plasmid PCR and restriction digestion
8/8: Extracted plasmid pBAR_RFP
8/13: pBAR_RFP checked with restriction digestion
8/13: pBAR_RFP checked with plasmid PCR
8/13: mRFP1 PCR amplification
8/13: TtrpC PCR amplification for pVP-EL222_RFP
8/14: pBAR_PgpdA plasmid stored and transformed cultures cryopreserved
8/14: C120_minimal PgpdA_RFP digested for the ligation with TtrpC
8/15: C120_minimal PgpdA_RFP_TtrpC transformed into competent cells
8/15: pBARGPE1 digested for pBAR_mRFP1_TtrpC construction
8/15: pVP-EL222 amplified with PCR
8/15: TtrpC and pVP- EL222 PCR amplification
8/16: TtrpC digestion
8/16: mRFP1 digestion
8/16: pBARGPE1 digested for pBAR_EL222_TtrpC construction
8/16: pBAR_EL222_TtrpC transformed into competent cells
8/16: pBAR_EL222_TtrpC containing cultures used 2 in 1
8/17: pBAR_EL222_TtrpC extracted and attempted to amplify with plasmid PCR (failure)
8/17: C120_minimal PgpdA_RFP_TtrpC extracted and checked with restriction digestion
8/17: C120_minimal PgpdA_RFP_TtrpC amplified with plasmid PCR
8/17: pBAR _RFP_TtrpC transformed into competent cells
8/17: pBAR _RFP_TtrpC containing cultures used in 2 in 1
8/18: pBAR _RFP_TtrpC did extracted from transformed cultures and checked with restriction digestion
8/18: C120_minimal PgpdA_RFP_TtrpC plasmid stored and transformed bacterial cultures cryopreserved
8/18: pBAR_PgpdA digested for the ligation with pVP-EL222 and TtrpC
8/18: pBAR_EL222_TtrpC transformed into competent cells
8/18: pBAR_EL222_TtrpC containing cultures used in 3 in 1
8/18: Amplified Mcl1 promoter (PMcl1) from genome (Taq PCR from Metarhizium anisopliae ARSEF549 genomic DNA)
8/19: pBAR _RFP_TtrpC checked with restriction digestion (the incorrect bands appeared in gel electrophoresis results)
8/19: mRFP1 PCR amplification (failed)
8/19: pBAR_EL222_TtrpC extracted from transformed cultures and checked with restriction digestion and plasmid PCR (failure)
8/19: pBAR_PgpdA_EL222_TtrpC transformed into competent cells
8/20: pVP-EL222 amplified with PCR and digested
8/21: pBAR_PgpdA_EL222_TtrpC extracted and checked with restriction digestion (failure)
8/21: mRFP1 PCR amplification
8/21: mRFP1 digestion
8/21: pBAR_PgpdA_EL222_TtrpC transformed into competent cells
8/22: PMcl1 Taq polymerase PCR amplification test run (failed, switched program afterwards)
8/23: PMcl1 KOD polymerase PCR amplification test run
8/23: PMcl1 Taq polymerase PCR amplification test run (with adjusted Mg2+ concentrations)
8/23: TtrpC PCR amplification
8/23: TtrpC digestion
8/23: pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1
8/23: BBa_E0040 containing cells T-streaked onto agar plates (failed to grow due to using incorrect antibiotics
8/24: pBAR_PgpdA_EL222_TtrpC checked with restriction digestion
8/24: pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR
8/24: PMcl1 genome PCR amplification test run
8/24: NahR_GFP resuspended (from 2015 NYMU iGem team’s cryopreservation bacterical cultures)
8/24: NahR_GFP containing cultures used in 2 in 1
8/25: PMcl1 PCR amplification test run
8/25: pVP-EL222 checked separately with plasmid PCR with redesigned primers
8/25: mRFP1 checked with PCR due to multiple failed double ligation
8/26: mRFP1 checked with KOD polymerase PCR
8/26: mRFP1 amplified with KOD polymerase PCR
8/26: pVP-EL222 checked with plasmid PCR due to multiple failed ligation
8/26: pVP-EL222 checked with restriction digestion due to multiple failed ligation
8/26: pVP-EL222 checked with Taq polymerase plasmid PCR with remixed primers
8/26: PMcl1 KOD polymerase PCR amplification test run
8/26: TtrpC KOD PCR check (with remixed pVP- EL222 primers)
8/27: TtrpC KOD PCR amplification
8/27: BBa_E0040 containing cells T-streaked onto agar plates
8/27: mRFP1 and TtrpC PCR ligation
8/27: pBARGPE1 checked with restriction digestion due multiple failed attempts to ligate pBARGPE1 with various inserts
8/27: pVP-EL222 checked with KOD polymerase plasmid PCR with remixed primers
8/27: pVP-EL222 amplified with PCR (use for the ligation with both pBAR_PgpdA and PMcl1)
8/28: RFP_TtrpC digested for ligation with pBARGPE1 (the concentration was not high enough)
8/28: mRFP1 and TtrpC did PCR to ligate(failure, we change the volume of the primer and Mg2+)
8/28: pVP-EL222 and TtrpC ran ligation PCR (NC had band)
8/28: pVP-EL222 and TtrpC ran ligation PCR (with remixed primer)
8/28: pVP-EL222 and TtrpC PCR ligation (NC had bands)
8/28: pVP-EL222 and TtrpC PCR ligation (used remixed primer)
8/29: pVP-EL222 and TtrpC PCR ligation (the concentration was not high enough after gel extraction)
8/29: pVP-EL222 and TtrpC ran ligation PCR (the concentration was not high enough after gel extraction)
8/31: pVP-EL222 and TtrpC ran ligation PCR
8/31: pBAR_RFP digestion(to transform into meta)
8/31: RFP_TtrpC digested for the ligation with pBARGPE1 (the band existed on wrong site)
8/31: pVP-EL222 and TtrpC ran ligation PCR
8/31: PMcl1 Taq polymerase PCR amplification
9/1: PMcl1 PCR amplification test run
9/1: PMcl1 KOD polymerase PCR amplification
9/1: PMcl1 polymerase PCR amplification
9/1: RFP_TtrpC digested
9/1: pVP-EL222 and TtrpC PCR ligate
9/1: EL222 and TtrpC ran ligation PCR
9/2: EL222-TtrpC digestion
9/2: pBARGPE1 digested for pBAR_EL222_TtrpC construction
9/2: pBAR _RFP_TtrpC transformed into competent cells
9/2: pBAR_PgpdA digested for ligation with EL222_TtrpC
9/2: PMcl1 KOD polymerase PCR amplification (failure)
9/2: PMcl1 KOD polymerase PCR amplification (failure)
9/3: PMcl1 KOD polymerase PCR amplification (failure)
9/3: pBAR_EL222_TtrpC transformed into competent cells
9/3: pBAR_EL222_TtrpC containing cultures used in 3 in 1
9/3: pBAR_PgpdA digested for ligation with EL222_TtrpC
9/3: pBAR_PgpdA_EL222_TtrpC transformed into competent cells
9/4: pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1
9/4: pBAR_PgpdA_EL222_TtrpC extracted from transformed cultures
9/4: pBAR _RFP_TtrpC did plasmid PCR
9/4: PMcl1 KOD polymerase PCR amplification
9/5: pBAR _RFP_TtrpC transformed into competent cells (there was no E.coli on the first plate)
9/5: mRFP1 amplified with KOD polymerase PCR
9/5: pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR
9/5: pBAR_EL222_TtrpC checked with restriction digestion
9/6: EL222-TtrpC digestion
9/6: pBAR_EL222_TtrpC checked with restriction digestion
9/6: pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (failure)
9/6: pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (we changed enzymes)
9/6: EL222-TtrpC checked with restriction digestion
9/6: EL222-TtrpC checked with restriction digestion
9/6: BBa_E0040 streaked onto agar plates
9/6: BBa_K118400 resuspended from 2016 iGEM Kit plate 4
9/6: BBa_K118400 transformed into competent cells
9/6: pBAR _RFP_TtrpC transformed into competent cells
9/6: PMcl1 KOD polymerase PCR amplification
9/6: PMcl1 KOD polymerase PCR amplification
9/7: PMcl1 KOD polymerase PCR amplification
9/7: pBAR _RFP_TtrpC transformed into competent cells
9/7: pBAR _RFP_TtrpC did 2 in 1
9/7: pBAR _RFP_TtrpC did 3 in 1
9/7: BBa_E0040 containing cultures used in 2 in 1
9/7: pBAR_EL222 PCR
9/7: BBa_K118400 containing culture used in 2 in 1
9/7: pVP-EL222 amplified with PCR (use for the ligation with both pBAR_PgpdA and PMcl1)
9/8: pVP-EL222 amplified with PCR (use for the ligation with both pBAR_PgpdA and PMcl1)
9/8: BBa_K118400 extracted from transformed cultures
9/8: BBa_K118400 amplified with plasmid PCR
9/8: BBa_E0040 extracted from transformed culture
9/8: pBAR _RFP_TtrpC checked plasmid PCR
9/8: pBAR _RFP_TtrpC checked with restriction digestion
9/8: pBAR _RFP_TtrpC digestion check
9/8: pBAR _RFP_TtrpC checked with plasmid extraction
9/8: PMcl1 KOD polymerase PCR amplification
9/9: PMcl1 KOD polymerase PCR amplification
9/9: pBAR _RFP_TtrpC checked with digestion check
9/9: BBa_E0040 checked with plasmid PCR
9/9: mRFP1 amplified with KOD polymerase PCR
9/9: BBa_E0040 checked with restriction digestion (successful)
9/9: BBa_K118400 checked with restriction digestion (the gel had faulty matrix)
9/9: BBa_K118400 checked with restriction digestion (confirmed successful)
9/9: BBa_K1184000 use KOD polymerase to amplify the target part KillerRed
9/9: pBAR_PgpdA_EL222_TtrpC checked with restriction digestion
9/9: pBAR_PgpdA_EL222_TtrpC transformed into competent cells (there was no E.coli on our first plate )
9/9: pBARGPE1 digested for pBAR_mRFP1_TtrpC construction
9/10: pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction
9/10: pBAR_PgpdA_EL222_TtrpC transformed into competent cells
9/10: BBa_K1184000 amplified with KOD polymerase to get the target part KillerRed
9/10: mRFP1 and TtrpC ran ligation PCR
9/10: RFP_TtrpC PCR to amplify the target sequence
9/10: Digested KillerRed fragment and its backbone pBARGPE1 with BamHI and EcoRI for the ligation to construct the vector pBAR_KR
9/10: Digested KillerRed fragment and its backbone pBARGPE1 with BamHI and EcoRI again
9/10: Amplified PMcl1 from genome (KOD polymerase)
9/10: Amplified PMcl1 from genome (KOD polymerase)
9/10: Standard part PgpdA amplification PCR with Taq polymerase
9/11: Standard part PgpdA amplification PCR with KOD polymerase
9/11: pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction
9/11: TtrpC KOD PCR amplification
9/11: TtrpC KOD PCR amplification
9/11: pBAR_PgpdA_EL222_TtrpC containing culture used in 3 in 1
9/11: pBAR_KR transformed into competent cell
9/11: pBAR_KR transformed into competent cell
9/12: pBAR_KR performed plasmid extraction and plasmid PCR (successful)
9/12: pBAR_PgpdA_EL222_TtrpC checked with restriction digestion
9/12: pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR
9/12: Successfully constructed the plasmid pBAR_PgpdA_EL222_TtrpC
9/12: Standard part PgpdA mutation PCR (mutated PstI)
9/12: Standard part PMcl1 amplification PCR with KOD polymerase
9/13: Standard Part PMcl1 mutation PCR (mutated SpeI)
9/13: Standard part TtrpC amplification PCR with KOD polymerase
9/13: pBAR _RFP_TtrpC transformed into competent cell
9/13: RFP_TtrpC PCR to amplify the target sequence
9/13: pBAR _RFP_TtrpC did 3 in 1
9/13: pBAR _RFP_TtrpC did digestion check
9/14: pBAR _RFP_TtrpC plasmid extraction
9/14: RFP_TtrpC digested
9/14: K118400 and TtrpC used PCR to amplify the target sequence
9/14: KR_TtrpC used PCR to amplify the target sequence
9/15: KR_TtrpC digested with restriction enzymes
9/15: Standard part TtrpC mutation PCR (mutated XbaI)
9/15: pBARGPE1 digested for pBAR_KR_TtrpC construction
9/16: pBAR_KR_TtrpC transformed into competent cells
9/16: Transformed pBAR_KR_TtrpC into competent cells
9/16: Digested pBAR_KR for transforming into Metarhizium anisopliae
9/16: Extracted plasmid BBa_K118400
9/16: pBAR_EL222_TtrpC did plasmid PCR
9/16: pBAR_EL222_TtrpC did plasmid PCR (confirmed successfully)
9/16: pBAR_RFP_TtrpC did digestion check
9/17: pBAR_RFP_TtrpC did digestion check
9/17: pBAR_EL222_TtrpC plasmid stored and transformed cultures cryopreserved
9/17: pBAR_KR_TtrpC did 3 in 1
9/17: Extracted plasmid pBAR_KR_TtrpC
9/18: Transformed pBAR_RFP_TtrpC into competent cells
9/18: pBAR_RFP_TtrpC did 3 in 1
9/18: pBAR_KR_TtrpC did plasmid PCR and digestion check
9/18: BBa_E0040 did plasmid PCR
9/19: Amplified GFP from plasmid BBa_E0040
9/19: Extracted plasmid BBa_E0040
9/20: KR_TtrpC digestion
9/20: Transformed pBAR_KR_TtrpC into competent cells
9/20: pBAR_KR_TtrpC containing cultures used in 3 in 1
9/20: Extracted plasmid pBAR_KR_TtrpC
9/20: pBAR_KR_TtrpC checked with restriction digestion
9/20: Extracted plasmid pBAR_RFP_TtrpC
9/20: pBAR_RFP_TtrpC did digestion check
9/20: RFP_TtrpC digestion
9/21: pBAR_KR_TtrpC checked with restriction digestion
9/21: pBAR_RFP_TtrpC checked with restriction digestion
9/21: pBAR_RFP_TtrpC containing cultures used in 2 in 1
9/21: pBARGPE1 and RFP_TtrpC digestion
9/22: Amplified RFP_TtrpC with KOD polymerase
9/22: Extracted plasmid pBAR_KR_TtrpC
9/22: pBAR_KR_TtrpC did plasmid PCR
9/22: PMcl1 and pBAR_KR_TtrpC digestion (PMcl1 was not digested successfully)
9/22: pBAR_PgpdA_EL222_TtrpC digested for transforming into Metarhizium anisopliae
9/23: Amplified PMcl1 with KOD polymerase
9/24: pBAR_RFP_TtrpC did digestion check
9/24: Extracted plasmid pBAR_RFP_TtrpC (from 9/5 plate)
9/25: Transformed pBAR_RFP_TtrpC into competent cell
9/25: RFP_TtrpC digestion
9/25: Amplified RFP-TtrpC with KOD polymerase
9/25: Amplified RFP_TtrpC with KOD polymerase
9/25: PMcl1 and pBAR_KR_TtrpC digestion
9/25: Transformed pBAR_PMcl1_KR_TtrpC into competent cell
9/25: Transformed pBAR_PMcl1_KR_TtrpC into competent cell
9/25: pBAR_PMcl1_KR_TtrpC did 3 in 1
9/26: Extracted plasmid pBAR_PMcl1_KR_TtrpC
9/26: pBAR_RFP_TtrpC containing cultures used in 2 in 1
9/26: Extracted plasmid pBAR_RFP_TtrpC
9/26: pBAR_RFP_TtrpC checked with restriction digestion
9/26: pBAR_RFP_TtrpC did digestion check
9/26: Amplified PMcl1 with KOD polymerase
9/27: Amplified PMcl1 with KOD polymerase
9/28: pBAR_PMcl1_KR_TtrpC did digestion check
9/28: pBAR_RFP_TtrpC did plasmid PCR
9/28: Successfully constructed the plasmid pBAR_RFP_TtrpC
9/28: Standard part TtrpC amplification PCR
9/29: Standard part TtrpC mutation PCR (mutated XbaI)
9/29: Standard part TtrpC mutation PCR (mutated XbaI)
9/29: Amplified PMcl1 with KOD polymerase
9/30: pBAR_PMcl1_KR_TtrpC did digestion check
9/30: Received the junction primer of GFP-TtrpC
9/30: GFP_TtrpC ran ligation PCR
9/30: Standard part TtrpC digestion
10/1: Standard part pSB1C3_TtrpC transformed into competent cells
10/1: pSB1C3 digestion
10/1: TtrpC digestion (for the ligation with pBARGPE1)
10/1: GFP_TtrpC ran ligation PCR
10/1 Amplified PMcl1 with KOD polymerase
10/1: Standard part RFP_TtrpC amplification PCR
10/1 Standard part RFP_TtrpC amplification PCR
10/2: Standard part RFP_TtrpC mutation PCR (mutated SpeI)
10/2: Standard part RFP_TtrpC mutation PCR (mutated SpeI)
10/2: Standard part RFP_TtrpC mutation PCR (mutated XbaI)
10/2: Amplified PMcl1 with KOD polymerase
10/2: PMcl1 digestion
10/2: GFP_TtrpC digested for ligation with pBARGPE1
10/2: Transformed pBAR_GFP_TtrpC into competent cells
10/2: pBARGPE1 digested for pBAR_GFP_TtrpC construction
10/2: pBARGPE1 resuspended (from cryopreservation)
10/2: Standard part PMcl1 amplification PCR
10/2: Standard part PMcl1 amplification PCR
10/2: Standard part pSB1C3_TtrpC containing cultures used in 2 in 1
10/3: Standard part pSB1C3_TtrpC plasmid extraction
10/3: Standard part pSB1C3_TtrpC checked with restriction digestion(failure)
10/3: Standard part PMcl1 amplification PCR
10/3: PMcl1 containing cultures used in 2 in 1
10/3: Extracted plasmid pBARGPE1
10/3: pBAR_GFP_TtrpC containing cultures used in 2 in 1
10/3: Extracted plasmid pBAR_GFP_TtrpC
10/3: pBAR_GFP_TtrpC digestion check(failure)
10/3: pBAR_GFP_TtrpC PCR check(failure)
10/3: Transformed pBAR_GFP_TtrpC into competent cells
10/3: pBARGPE1 checked with restriction digestion (correct)
10/3: Standard part RFP_TtrpC amplification PCR
10/3: Standard part RFP_TtrpC amplification PCR
10/4: Standard part RFP_TtrpC amplification PCR
10/4: pBARGPE1 plasmid stored
10/4: pBAR_GFP_TtrpC containing cultures used in 2 in 1
10/4: pBAR_GFP_TtrpC plasmid extraction
10/4: pBAR_GFP_TtrpC digestion check
10/4: PMcl1 digestion for ligation with pBAR_KR_TtrpC
10/4: pBAR_KR_TtrpC digestion
10/4: Transformed pBAR_ PMcl1 _KR_TtrpC into competent cells
10/4: Standard part PgpdA amplification PCR
10/4: Standard part PgpdA mutation PCR (mutated PstI and EcoRI)
10/4: Standard part PMcl1 mutation PCR (mutated XbaI)
10/4: Standard part pSB1C3_TtrpC transformed into competent cells
10/4: Standard part pSB1C3_TtrpC containing cultures used in 2 in 1
10/5: Standard part pSB1C3_TtrpC plasmid extraction
10/5: Standard part pSB1C3_TtrpC digestion check (correct)
10/5: Standard part pSB1C3_TtrpC amplified with plasmid PCR
10/5: Standard part PMcl1 amplification PCR
10/5: Standard part PMcl1 amplification PCR
10/5: Standard part pSB1C3_PgpdA transformed into competent cells
10/5: Standard part pSB1C3_PgpdA containing cultures used in 2 in 1
10/5: pBAR_GFP_TtrpC checked with restriction digestion
10/5: Transformed pBAR_GFP_TtrpC into competent cells
10/5: pBAR_RFP_TtrpC digestion (for the ligation with PMcl1)
10/5: pBAR_ PMcl1 _KR_TtrpC containing cultures used in 2 in 1
10/5: Standard part RFP_TtrpC amplification PCR
10/5: Standard part RFP_TtrpC mutation PCR (mutated SpeI)
10/6: Standard part RFP_TtrpC mutation PCR (mutated SpeI)
10/6: Standard part RFP_TtrpC mutation PCR (mutated XbaI)
10/6: Extracted plasmid pBAR_PMcl1_KR_TtrpC
10/6: pBAR_PMcl1_KR_TtrpC checked with restriction digestion
10/6: pSB1C3 digestion from 2015 iGem kit
10/6: Extracted plasmid pSB1C3_PgpdA
10/6: Standard part pSB1C3_PgpdA plasmid PCR
10/6: Standard part pSB1C3_PgpdA digestion check
10/7: Standard part pSB1C3_PgpdA checked with restriction digestion
10/7: Standard part PgpdA amplification PCR
10/7: pSB1C3 digestion
10/7: Standard part RFP_TtrpC checked with KOD polymerase
10/7: pBAR_GFP_TtrpC did digestion check (failure)
10/8: pBAR_PMcl1_KR_TtrpC checked with restriction digestion (failure)
10/8: pBAR_RFP_TtrpC digestion
10/8: Transformed pBAR_PMcl1_RFP_TtrpC into competent cells
10/8: Standard part PgpdA mutation PCR (mutated PstI and EcoRI)
10/8: Standard part pSB1C3_PgpdA transformed into competent cells
10/8: Standard part PMcl1 amplification PCR
10/9: Standard part PMcl1 amplification PCR
10/9: Standard part pSB1C3_PgpdA containing cultures used in 2 in1
10/9: Standard part pSB1C3_PgpdA did digestion check
10/9: pBAR_PMcl1_RFP_TtrpC containing cultures used in 2 in 1
10/9: Extracted plasmid pBAR_PMcl1_RFP_TtrpC
10/9: pBAR_PMcl1_RFP_TtrpC checked with restriction digestion
10/9: pBAR_PMcl1_RFP_TtrpC checked with restriction digestion
10/9: pBAR_PMcl1_RFP_TtrpC checked with restriction digestion
10/9: Standard part RFP_TtrpC checked with KOD polymerase
10/10: Standard part RFP_TtrpC amplification PCR
10/10: pBAR_PMcl1_RFP_TtrpC checked with restriction digestion (failure)
10/10: Standard part pSB1C3_PgpdA plasmid extraction
10/10: Standard part pSB1C3_PgpdA checked with restriction digestion (confirmed to be correct)
10/10: Standard part PgpdA mutation PCR (mutated PstI and EcoRI)
10/10: Standard part pSB1C3_PgpdA amplified with plasmid PCR (correct)
10/10: Standard part PMcl1 amplification PCR
10/11: Standard part PMcl1 mutation PCR (mutated XbaI)
10/11: Standard part RFP_TtrpC amplification PCR
10/11: Standard part RFP_TtrpC amplification PCR
10/11: Standard part RFP_TtrpC mutation PCR (mutated SpeI)
10/12: Standard part RFP_TtrpC mutation PCR (mutated XbaI)
10/12: Standard part RFP_TtrpC digestion
10/12: Standard part pSB1C3_PgpdA stored plasmid
10/12: Standard part PMcl1 mutation PCR (mutated SpeI)
10/12: Standard part KR_TtrpC amplification PCR
10/12: Standard part minPgpdA amplification PCR
10/13: Standard part minPgpdA amplification PCR
10/13: Standard part KR_TtrpC amplification PCR
10/13: Standard part Pmcl1 mutation PCR (mutated SpeI)
10/13: Standard part pSB1C3_RFP_TtrpC transformed into competent cells
10/13: Standard part pSB1C3_RFP_TtrpC containing cultures used in 2 in 1
10/13: Standard part pSB1C3_RFP_TtrpC did digestion check
10/13: Standard part pSB1C3_RFP_TtrpC amplified with plasmid PCR
10/14: Standard part pSB1C3_RFP_TtrpC checked with restriction digestion
10/14: KR_NLS amplification PCR
10/14: Standard part minPgpdA amplification PCR
10/15: Standard part minPgpdA amplification PCR
10/15: Standard part minPgpdA amplification PCR
10/15: Standard part minPgpdA digestion
10/15: pSB1C3_RFP_TtrpC checked with restriction digestion
10/15: Standard part pSB1C3_minPgpdA transformed into competent cells (failure)
10/15: Standard part RFP_TtrpC did 2 in 1
10/16: Standard part pSB1C3_minPgpdA transformed into competent cells
10/16: Standard part RFP_TtrpC checked with restriction digestion
10/16: Standard part pSB1C3_minPgpdA containing cultures used in 2 in 1
10/16: Standard part BBa_K118400 digestion
10/16: Standard part pSB1C3_KR transformed into competent cells
10/16: Standard part minPgpdA amplification PCR
10/16: Standard part RFP_TtrpC plasmid extraction
10/16: Standard part RFP_TtrpC checked with plasmid PCR
10/16: Standard part RFP_TtrpC did digestion check
10/17: Standard part pSB1C3_KR did 2 in 1
10/17: Standard part minPgpdA amplification PCR
10/17: Standard part pSB1C3_KR plasmid extraction
10/17: Standard part pSB1C3_KR checked with restriction digestion
10/17: Standard part PgpdA digestion
10/17: pSB1C3 digestion
10/17: Standard part pSB1C3_minPgpdA transformed into competent cells
10/17: Standard part pSB1C3_minPgpdA plasmid extraction
10/17: Standard part pSB1C3_minPgpdA checked with plasmid PCR (correct)
10/18: Standard part pSB1C3_minPgpdA checked with digestion check (correct)
10/18: Standard part PgpdA_KR_TtrpC amplification PCR
10/18: Standard part KR_TtrpC mutation PCR (mutated EcoRI)
10/18: Standard part KR_TtrpC digestion
10/18: Standard part pSB1C3_KR_TtrpC transformed into competent cells
10/18: Standard part pSB1C3_KR_TtrpC containing cultures used in 3 in 1
10/18: Standard part PgpdA_KR_TtrpC amplification PCR
10/19: Standard part pSB1C3_KR_TtrpC plasmid extraction
10/19: Standard part PgpdA_KR_TtrpC mutation PCR (mutated XbaI)
10/19: Standard part PgpdA_KR_TtrpC mutation PCR (mutated EcoRI)
10/19: Standard part pSB1C3_PgpdA_KR_TtrpC transformed into competent cells
10/20: Standard part pSB1C3_PgpdA_KR_TtrpC containing cultures used in 3 in 1
10/20: Standard part pSB1C3_PgpdA_KR_TtrpC plasmid extraction
10/20: Standard part pSB1C3_KR_TtrpC plasmid extraction
10/20: Standard part pSB1C3_KR_TtrpC checked with restriction digestion
10/20: Standard part pSB1C3_KR_TtrpC checked with plasmid PCR
10/20: Standard part pSB1C3_PgpdA_KR_TtrpC checked with plasmid PCR
10/20: Standard part pSB1C3_PgpdA_KR_TtrpC checked with restriction digestion
