Team:NYMU-Taipei/Notebook-Lab Book-RFP

RFP

RFP


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7/9

  • BBa_E1010 T-streaked onto agar plates

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    7/10

  • BBa_E1010 extracted plasmid and checked with restriction digestion (EocRI and PstI 747bp and 2029bp)

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    7/16

  • BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)

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    7/17

  • BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)

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    7/18

  • BBa_E1010 checked with plasmid PCR with newly design primers

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    7/19

  • BBa_E1010 checked with plasmid PCR with the addition of Mg2+

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    7/22

  • BBa_E1010 checked with Taq polymerase plasmid PCR (successful)

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    7/25

  • BBa_E1010 amplified with KOD polymerase PCR

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    7/26

  • RFP amplified with KOD polymerase PCR

  • RFP digested with EcoRI and BamHI

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    7/29

  • pBAR_RFP checked with KOD polymerase PCR (contaminated NC)

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    8/1

  • RFP checked with KOD PCR

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    8/3

  • RFP digested with EcoRI and BamHI for the ligation with C120_empty

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