Team:NYMU-Taipei/Notebook-Lab Book-mRFP1

mRFP1

mRFP1


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7/9

  • BBa_E1010 T-streaked onto agar plates

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    7/10

  • BBa_E1010 extracted the checked with restriction digestion (EocRI and PstI, 747bp and 2029bp)

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    7/16

  • BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)

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    7/17

  • BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)

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    7/18

  • BBa_E1010 checked with plasmid PCR with newly design primers

    • 7/19

  • BBa_E1010 checked with plasmid PCR with the addition of Mg2+

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    7/22

  • BBa_E1010 checked with Taq polymerase plasmid PCR (successful)

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    8/13

  • mRFP1 PCR amplification

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    8/16

  • mRFP1 digestion

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    8/19

  • mRFP1 PCR amplification (failed)

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    8/21

  • mRFP1 PCR amplification

  • mRFP1 digestion

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    8/25

  • mRFP1 checked with PCR due to multiple failed double ligation

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    8/26

  • mRFP1 checked with KOD polymerase PCR

  • mRFP1 amplified with KOD polymerase PCR

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    9/5

  • mRFP1 amplified with KOD polymerase PCR

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    9/9

  • mRFP1 amplified with KOD polymerase PCR

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