Team:NYMU-Taipei/Notebook-Lab Book-minimal-PgpdA

minimal PgpdA

minimal PgpdA


[Collapse all] | [Expand all]

7/22

  • Transformed plasmid pAN7-1 into competent cells

  • plasmid pAN7-1 containing cultures used in 2 in 1 (liquid culture & second plate)

    • [Collapse]

    7/23

  • Extracted plasmid pAN7-1

    • [Collapse]

    7/24

  • plasmid pAN7-1 checked with plasmid PCR

  • plasmid pAN7-1 stored plasmid and cryopreservation bacterical cultures

    • [Collapse]

    7/26

  • Amplified minimal PgpdA promoter on pAN7-1 by doing KOD PCR

    • [Collapse]

    7/28

  • Digested minimal PgpdA fragment for the ligation with pC120_empty backbone to construct vector pC120_minPgpdA

    • [Collapse]