Wednesday, June 22nd
Agenda:
- Check over the GenScript grant
- Update experiment presentation
- Research Questions
- Troubleshooting the transformation
- iGEM safety sheets
- FITC dilutions
- Website CSS
Tasks:
Paul & Sara
- Troubleshooting the transformation
- Followed the competent cell protocol from the third boot camp with the cells we grew overnight
- Placed 5 mL LB and 50 uL of cell culture into each Falcon tube, then placed in the incubator for ~3 hours
- After making competent cells, we followed the iGEM transformation protocol using the competent cells made that day, competent cells that Kelly brought us as a control, and control competent cells with kanamycin resistance
- With these controls, we should get a better idea of whether the transformation failure was due to the DNA volume typo on the iGEM page, bad chloramphenicol, or badly made competent cells.
- Incubated overnight
Sam & Tasfia
- FITC dilutions
- The Ludox from the iGEM interlab kit was not suspended properly
- Absorbance was 1.2 instead of 0.4
- Emailed iGEM HQ asking them to resend the Ludox
Michelle
- Researched questions that Patrick pointed out, ClyA and LPS toxicity, as well as where in/on the body OMVs can be applied
- Worked on the website
- Helped with the plate reader
Shu
- Worked on the team logo
Tyler
- Worked with Genome Compiler and looked into designing plasmids
- Emailed sponsors
- More background research
- Meeting with sponsor at Fri. 10:30
Sam
- Did the dilutions
- Helped with the Genscript grant
Jordan
- Emailed CTD to set up outreach opportunities
- Set up lab tours for high school kids on July 8, Mike and Josh’s labs
- Helped plate
Paul & Sara
- Troubleshooting the transformation
- Followed the competent cell protocol from the third boot camp with the cells we grew overnight
- Placed 5 mL LB and 50 uL of cell culture into each Falcon tube, then placed in the incubator for ~3 hours
- After making competent cells, we followed the iGEM transformation protocol using the competent cells made that day, competent cells that Kelly brought us as a control, and control competent cells with kanamycin resistance
- With these controls, we should get a better idea of whether the transformation failure was due to the DNA volume typo on the iGEM page, bad chloramphenicol, or badly made competent cells.
- Incubated overnight
Sam & Tasfia
- FITC dilutions
- The Ludox from the iGEM interlab kit was not suspended properly
- Absorbance was 1.2 instead of 0.4
- Emailed iGEM HQ asking them to resend the Ludox
Michelle
- Researched questions that Patrick pointed out, ClyA and LPS toxicity, as well as where in/on the body OMVs can be applied
- Worked on the website
- Helped with the plate reader
Shu
- Worked on the team logo
Tyler
- Worked with Genome Compiler and looked into designing plasmids
- Emailed sponsors
- More background research
- Meeting with sponsor at Fri. 10:30
Sam
- Did the dilutions
- Helped with the Genscript grant
Jordan
- Emailed CTD to set up outreach opportunities
- Set up lab tours for high school kids on July 8, Mike and Josh’s labs
- Helped plate
