Thursday, June 30th
Agenda:
- Constructs
- Discuss things with Purdue
- Protocol Practice- miniprep and digestion
- Crowdfunding
- Presentation practice
Results:
- Nothing in the gel except the ladder was visible under UV light
- The ImageJ program was not working
- Left the gel out too long in the light and our SybrGreen degraded
Tasks:
Michelle
- CSS studying
- Tat & Sec sequence selection
- Miniprepped practice plasmids
Sam
- Wrote about things to discuss with Purdue
Sara
- Did the digest and ran the gel
- Emailed Bernd about split Cas9
Tasfia
- ClyA construct
- Miniprep practice
Tyler
- Communicated w/sponsors (Promega and Invitrogen)
- Worked on split Cas9 project, designed overlaps, need to check with Bradley
Michelle
- CSS studying
- Tat & Sec sequence selection
- Miniprepped practice plasmids
Sam
- Wrote about things to discuss with Purdue
Sara
- Did the digest and ran the gel
- Emailed Bernd about split Cas9
Tasfia
- ClyA construct
- Miniprep practice
Tyler
- Communicated w/sponsors (Promega and Invitrogen)
- Worked on split Cas9 project, designed overlaps, need to check with Bradley