Tuesday, July 5th
Agenda:
- Meet with Taylor to make legit comp cells (11 am)
- Skype the Purdue iGEM team (1 pm)
- Use the plate reader for the Interlab
- Make Golden Gate primers
- Work on Gibson primers and overhangs
Tasks:
Jordan
- Made competent cells with Taylor
- Crowdfunding stuff
- Made Experiment centrifuge video
Michelle
- Prepared competent cells aliquots with Taylor
- Website reading
- Finished ClyA homology
- Created signaling sequence homology
- Checked existing primers and constructs
Paul
- Designed GFP Golden Gate Primers
- Designed GFP flexible linker primers
- Communicated w/ Purdue
- Got familiar w/ Genome Compiler
Sam
- Plate reader for Interlab
- Researched potential outreach people
- Researched Gibson assembly
- Talked to Purdue
- Mosquito forum stuff for Building with Biology outreach
Sara
- Worked on Golden Gate constructs
- Went to the Skype call with Purdue
Tasfia
- ClyA plasmid and homology
- Prepared competent cells that hopefully actually work this time
- Updated slideshow
- Researched Golden Gate assembly
Tyler
- E-mailed Feinberg about free sequencing
- Made slight changes to split Cas9 sequence
- Generated Primers for opening plasmid at SS (have to redo tomorrow because change in RBS sequence)
- Reviewed signal sequences with homology arms
Jordan
- Made competent cells with Taylor
- Crowdfunding stuff
- Made Experiment centrifuge video
Michelle
- Prepared competent cells aliquots with Taylor
- Website reading
- Finished ClyA homology
- Created signaling sequence homology
- Checked existing primers and constructs
Paul
- Designed GFP Golden Gate Primers
- Designed GFP flexible linker primers
- Communicated w/ Purdue
- Got familiar w/ Genome Compiler
Sam
- Plate reader for Interlab
- Researched potential outreach people
- Researched Gibson assembly
- Talked to Purdue
- Mosquito forum stuff for Building with Biology outreach
Sara
- Worked on Golden Gate constructs
- Went to the Skype call with Purdue
Tasfia
- ClyA plasmid and homology
- Prepared competent cells that hopefully actually work this time
- Updated slideshow
- Researched Golden Gate assembly
Tyler
- E-mailed Feinberg about free sequencing
- Made slight changes to split Cas9 sequence
- Generated Primers for opening plasmid at SS (have to redo tomorrow because change in RBS sequence)
- Reviewed signal sequences with homology arms
