Team:Northwestern/07 30


Saturday, July 30th


The Gibson reaction plates have no visible colonies.


Sara & Shu

  • Ran mCherry colonies along with one PCR reaction from the first attempt at colony PCR (m1' and G1')
    • Ran 3 controls:
      • No DNA- the PCR reaction contained everything but template DNA
      • Positive control- DNA straight from the iGEM kit containing RFP, which is a similar size to our desired inserts (J04450 in pSB1K3)
      • Backbone- Which was only the pSB1K3 backbone
  • Figure 1: mCherry PCR products

    Figure 2: GFP PCR products