Saturday, July 30th
Results:
The Gibson reaction plates have no visible colonies.
Tasks:
Sara & Shu
- Ran mCherry colonies along with one PCR reaction from the first attempt at colony PCR (m1' and G1')
- Ran 3 controls:
- No DNA- the PCR reaction contained everything but template DNA
- Positive control- DNA straight from the iGEM kit containing RFP, which is a similar size to our desired inserts (J04450 in pSB1K3)
- Backbone- Which was only the pSB1K3 backbone
- Ran 3 controls:
Figure 1: mCherry PCR products
Figure 2: GFP PCR products
