Team:Northwestern/08 06

Notebook

Saturday, August 6th

Tasks:

Sara & Tyler

  • Checked and made a list of the plates in the incubator, grew up colonies of Cas9 and GFP storage vectors, 3 mL of LB, no antibiotics

    • Figure 1: Cas9 1:2 Gibson

    Figure 3: Cas9 1:3 Gibson 2

    Figure 5: GFP 2 storage vector

    Figure 7: No ligase GFP control

    Figure 2: Cas9 1:3 Gibson

    Figure 4: GFP 1 Storage vector

    Figure 6: GFP 3 storage vector

  • Colonies on ligase control indicate problem with our Tet plates