Tuesday, September 27th
Tasks:
Jordan
- DpnI digest of SS-Cas9 PCR
Paul
- PCR of all SS-Cas9 with new primers
Sara
- Ran a gel screen on Cas9 and SS with restriction digest ends for assembly into pSB1C3 plasmid
- It did not work
Tasfia
- Retransform all pET28a Parts (edit: just use colonies from last time, using a combination of colony pcr and restriction digest to figure out which colonies match up with which sequencing prep)
- Grow up cultures of pET28a parts
Tyler
- Pick Up Primers
- Take cas9+gRNA cultures out of shaking incubator
Jordan
- DpnI digest of SS-Cas9 PCR
Paul
- PCR of all SS-Cas9 with new primers
Sara
- Ran a gel screen on Cas9 and SS with restriction digest ends for assembly into pSB1C3 plasmid
- It did not work
Tasfia
- Retransform all pET28a Parts (edit: just use colonies from last time, using a combination of colony pcr and restriction digest to figure out which colonies match up with which sequencing prep)
- Grow up cultures of pET28a parts
Tyler
- Pick Up Primers
- Take cas9+gRNA cultures out of shaking incubator
