Friday, September 30th
Tasks:
Jordan
- Transform SS-Cas9 into Delisa cells based off which worked (could push this back to Saturday/Sunday/Monday)
- Gel/DpnI/PCR Purify TorA
- Restriction digest of TorA-Cas9, YcdO-Cas9, and DsbA-Cas9 into pSB1C3 bacbone
- Ligate
- Transform RD product
Jordan
- Transform SS-Cas9 into Delisa cells based off which worked (could push this back to Saturday/Sunday/Monday)
- Gel/DpnI/PCR Purify TorA
- Restriction digest of TorA-Cas9, YcdO-Cas9, and DsbA-Cas9 into pSB1C3 bacbone
- Ligate
- Transform RD product
