Team:TCU Taiwan/Basic Part

Name	Type	Description	Length
Parts table
BBa_K2011000	Coding	sgrS binding site	255 b.p
BBa_K592012	Coding	Red Chromoprotein	681 b.p
BBa_k2011408	Coding	sgrS binding site with red chromoprotein	936 b.p
BBa_K2011002	Composite	T5 Promoter + RBS + sgrS binding site with Red Chromoprotein	936 b.p

Name	Type	Description	Length
Basic parts table
BBa_K2011000	Coding	sgrS binding site	255 b.p
BBa_K592012	Coding	Red Chromoprotein	681 b.p
BBa_k2011408	Coding	sgrS binding site with red chromoprotein	936 b.p


SgrS BS: The SgrS BS sequence consist of ptsG sequence, the transcription of the ptsG sequence will produce ptsG mRNA which this mRNA will help to the production of glucose transporter IICB_glc , this will help the uptake of glucose . But the mechanism of ptsG to become IICB_glc can be blocked by the presence of the SgrS. SgrS can be produced by the help of the SgrR when the concentration of glucose is high

Red color chromoprotein: It's a Fluorescent gene which expresses red color once the gene is expressed. (From iGEM11_Uppsala-Sweden)

SgrS BS + RED color chromoprotein Once the recombinant gene is expressed: (In the presence a lot of glucose) SgrS will bind with the sequence of recombinant mRNA , and it will degrade the activation of ptsG with the help of RNase and another cofactor, resulting in no red color produced because it's also bind with the ptsG sequence. (In the presence of low glucose) SgrS will be produced in low level and the number of SgrS is not effective enough to degrade the presence of the ptsG sequence , resulting red color will be still produced.

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