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Week 4: June 10 2016

Friday, 6/10

Members Present: Kat, Marc, Alex, Cathy, Hamed, Dk

LAB:

Morning:

- Made TSS buffer

- Grew cells to OD600 of 0.34 (please double-check the hard-copy)

Afternoon:

- Made aliquotes of chemically competent cells

- Created small nickel plasmids

Administrative:

- Provided feedback on CSM presentation

- Obtained freezer space.

- Labelled drawers and files (protocols and MSDS)

- Weekly inventory completed

- E-mailed GE Healthcare Life Science regarding HiTrap Heparin column protocol, their response were as follows:

Hi Zarifah,

The column can be operated with a syringe, peristaltic pump or a chromatography system. It is much easier to control the flow rate, running and maximum with either a peristaltic pump or a chromatography system. If one is careful a syringe can work fine.

Regards,

Charlie

TO DO:

For the next day:

LAB TEAM:

- Submit IDT order

- Measure transformation efficiency

LAB MANAGERS:

- Speak to Susie regarding unknown waste

- Pickup MedStore order (quote order number 340173) - disposable inoculation loops

- Purchase PCR tubes, P200 tips, small gloves

Team:Toronto/Notebook-w04-fri

Week 4: June 10 2016