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Week 4: June 6 2016

Monday, 6/6

Members present: Kat, Dk, Marc, Alex, Cathy, Hamed

Lab Stuff: (research, plasmid design, protocol design, troubleshooting)

Protocols to sort out:

- Antibiotic stocks (CAM)

- Media (LB [agar + liquid] - need to have clean glassware

- Competent Cells (TSS buffer)

- Transformation Efficiency Kit (basically transformation)

- Miniprep afterwards

- Nanodrop

Sorted out the -20C freezer

- only partially, the box containing LCR+Gibson+Synbiota reagents and products

Administrative: (shipment orders, inventory)

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Participated in the annual BioZone lab cleaning from 9.30 to 12 noon

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Updated inventory to include physical consumables - Monday, 6th June

Email Updates: (Correspondence with professors, shipping companies, etc)

Meeting Notes:

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Meeting with Sean Caffrey

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https://docs.google.com/document/d/1GopeSGqn5a1mBVCHD6nYrhgSttdXN8eHzdi1fcmXEfc/edit

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Meeting with Naveen:

Things to Do:

- Redo RBSs recalculated for 200,000 T.I.R. as target

- Design primers for LCR

- Check if IDT includes primers

- Submit plasmids to IDT (but not order yet) to make sure they are doable

- Design LCR backbone with UNS's attached along with primers (Naveen's design #3)

- Redo restriction sites with PstI instead of SpeI (including backbone extensions)

- Before submitting to IDT, ask if Naveen has the primers

Schedule for Week

	A	B	C	D
1	Tuesday	Wednesday	Thursday	Friday
2	- Antibiotic Stocks	- Glycerol stocks	-Transformation efficiency	-Restriction enzyme digest (PstI + SpeI)
3	- Media (SOC, LB, LB + Agar)	- Competent cells	- Miniprep	- Gel Electrophoresis
4	- Grow out cells	-Transformation efficiency (if time)	- Nanodrop

Table1

Team:Toronto/Notebook-w04-mon