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Friday July 8, 2016

Friday, 7/8

bMembers Present: DK, Alex, Cathy, Tam, Hamed, Kat

LAB:

Morning:

- Made S30 Wash Buffer (A):

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450mL filter sterilized

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550mL non-sterilized

Afternoon:

- Took photos of our cultures in the gel doc

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only obvious successful transformations were with GolS and GolP118A(Note: there is a reddish tinge to these; may be the result of contamination)

BL21 Culture on Agar: confirmed that we are not growing yeast (to rule out contamination from shaker)

CCD cells transformed with ligated Long Linear GolS (10 colonies)

CCD cells transformed with ligated Long Linear GolSP118A (2 colonies)

CCD cells transformed with mCherry (0 colonies)

CCD cells transformed with RFP (control)

TO DO:

For the next day:

LAB TEAM:

- do small cultures of single successful transformed colonies

- miniprep

- PCR all inserts and run a gel on them

- Make 1L of 2xYTPG

- Pick a single colony of BL21 and grow a 5mL culture over night

Team:Toronto/Notebook-w08-fri