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Thursday - July 21

Friday, 7/22

Members Present: Hamed, Marc, Celine, DK, Kat

LAB:

Morning:

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More PCR of pSB1C3 backbone

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Gel of PCR's backbone:

PCR amplified samples of the pSB1C3 backbone. This is for future attempts of gel extraction.

Afternoon:

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Re Digest of LacZ insert, and of backbone from the kit (linear PCR amplified already) and backbone amplified from the RFP plasmid

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Split the digested backbone into two, and dephosphorylated half of them with rSAP.

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Kit pSB1C3 - LacZ

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Kit pSB1C3 (rSAP) - LacZ

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RFP pSB1C3 - LacZ

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RFP pSB1C3 (rSAP)- LacZ

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PCR purification and nanodrop of the samples.

Table1

Note that the +/- refers to wether or not rSAP was used

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Overnight ligation of the samples at 16C

Team:Toronto/Notebook-w10-thu