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Friday, August 5th

Friday, 8/5

Members Present: Kat, Cathy, Tam, DK, Hamed

LAB:

Morning:

- PCR purification of PCR-amplified parts

Afternoon:

- dpnI digest of PCR-purified backbone

- EcoRI HF and PstI HF digest of inserts

TO DO:

For the next day:

LAB TEAM:

- PCR purify all digested parts (combining two tubes into one to increase concentration)

- EcoRI HF anad PstI HF digest of backbone after PCR purification

LAB MANAGERS:

- Make sure more linearized backbone has been ordered from iGEM (at least 50ul tubes of pSB1C3)

- Follow up with Susie to make sure Amino acids have been ordered

Team:Toronto/Notebook-w12-fri