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Wednesday, August 3
Wednesday, 8/3
Members Present:
LAB:
Morning:
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Miniprep of lacZ ligation O/N cultures
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Nanodrop of miniprepped ligations:
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RE single digest of miniprepped lacZ ligations, as well as Kayla's pCOLA ligations
Afternoon:
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Made more rubidium chloride competent cells (both DH10B and BL21), labelled CCE and CC Bl21, respectively
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Ran gradient PCR on Long GolS (G 1-6) , Long GolS P118A (P 1-6), mCherry (C 1-6).
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Ran 3x15 lane gels on RE digested lacZ constructs, pCOLA, and on PCR gradients
TO DO:
For the next day:
LAB TEAM:
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PCR of backbone
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PCR purification of backbone and constructs pcr amplified today - GolS, p118A, mCherry)
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Vacufuge some of the larger constructs to an appropriate conc for digest (ask andy for advice)
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RE digest of constructs and backbone
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rSAP of backbone
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PCR purification
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Ligation O/N at 16C
LAB MANAGERS:
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Some autoclaving would be noice