Team:Toronto/Notebook-w16-wed
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Wednesday, August 31
Wednesday, 8/31
Members Present:
LAB:
Morning:
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Miniprepped cultures made yesterday (as well as LacZ from the day before):
A | B | C | D | |
1 | Sample | 260/280 | 260/230 | conc (ng/uL) |
2 | LacZ | 1.81 | 1.36 | 106.9 |
3 | SP | 1.9 | 1.57 | 95.3 |
4 | SG | 1.69 | 0.98 | 150.3 |
5 | P | 1.78 | 1.17 | 81.7 |
6 | 1 | 1.74 | 0.92 | 51.9 |
7 | 2 | 1.68 | 0.92 | 63.5 |
Miniprep - August 31st
Nore that 1 and 2 were used to denote the O/N cultures that were in the fridge, as their labels had worn off
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Made O/N cultures (5ml) of the remainder for further miniprep
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Made O/N cultures from the transformants from the previous day. SG was unsuccessful, therefore it was transformed once more
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Made aliquots of nf H20
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Made more LB + CAM plates
Afternoon:
Administrative:
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Talked to Kayla, she promised to send us the junk DNA for our LacZ omega construct
TO DO:
For the next day:
LAB TEAM:
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Gel extract the constructs that were unsuccessful
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Miniprep the O/N cultures.
LAB MANAGERS:
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Buy more falcon tubes
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FInd where our missing p200 is
Gmail correspondence:
Meetings/Notes:
References:
